AB286217

Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric)

20ul selling size
Recombinant
What is this?

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Human Recombinant Monoclonal NCAP antibody. Suitable for WB, I-ELISA and reacts with SARS-CoV-2 samples.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

2 Images

Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric) (AB286217)

I-ELISA

Lab

Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric) (AB286217)

Indirect ELISA- Anti-SARS-CoV-2 nucleocapsid protein antibody (ab286217). Indirect ELISA showing primary antibody ab286217 binding to recombinant human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530). Plates were coated with recombinant human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) at 1000 ng/ml. Binding of ab286217 was assessed in a serial dilution range 0.045- 100 ng/mL (a 3-fold serial dilution).
Binding was detected using goat anti-human IgG Fc (HRP) preadsorbed (ab98624) at 1/1000 dilution.

Lab

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric) (AB286217)

Green - ab286217 observed at 50 kDa. ab286217 was shown to react with SARS-CoV-2 nucleocapsid in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween ® ) before incubation with ab286217 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Human 800CW secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric) (ab286217)

All lanes:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>)

Secondary

All lanes:

Goat anti-Human 800CW at 1/20000 dilution

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Key facts

Host species

Human

Clonality

Monoclonal

Clone number

F12

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

SARS-CoV-2

Applications

I-ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "0.001-1 µg/mL", "IELISA-species-notes": "<p></p>" } } }

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Product details

ab286217 is a human monoclonal chimeric antibody.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

See full target information N

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric)

Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric) (AB286217)

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric) (AB286217)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise