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AB281295

Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free

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Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ICC, WB, sELISA, IHC-P, I-ELISA and reacts with SARS-CoV-2 samples.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)

Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained by ab281295 (green) at 1/1000 dilution in a immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)

Mock and transfected SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid stained by ab281295 (green) at 1/1000 dilution in immunocytochemistry analysis. Fluoroshield with DAPI (blue).

Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281302 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281295 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281295 needs to be conjugated to HRP to function as the detection antibody when paired to ab281302.

Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (A and B) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with ab281295 at 1 μg/mL. HRP-conjugated Rabbit IgG antibody was diluted at 1/10000 and used to detect the primary antibody.

Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281295 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody (1 : 10000) detected bound primary antibody.
EC50 : 0.016 nM

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295) at 1/5000 dilution

Lane 1:

293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

SARS-CoV NP transfected 293T whole cell extracts at 30 µg

Lane 3:

SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts at 30 µg

Lane 4:

MERS-CoV transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (AB281295)

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295) at 1/5000 dilution

Lane 1:

293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts at 30 µg

Secondary

Lane 1:

HRP-conjugated anti-rabbit IgG

Lane 2:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL249

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, sELISA, ICC, IHC-P, I-ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/1000", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "5 µg/mL", "sELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
See full target information N
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