Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)

Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for IHC-P, ICC, WB, sELISA, I-ELISA and reacts with SARS-CoV-2 samples.

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Images

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (AB281296), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC	WB	sELISA	I-ELISA
SARS-CoV-2	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info 1/100.00000 - 1/1000.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info 1/5000.00000 - 1/20000.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info 5 µg/mL	Notes -

Tested
Tested

Species	Dilution info	Notes
Species SARS-CoV-2	Dilution info 1 µg/mL	Notes -

Target data

See full target information N

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661). May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414). May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

Alternative names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

Recommended products

Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for IHC-P, ICC, WB, sELISA, I-ELISA and reacts with SARS-CoV-2 samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Carrier free: Yes
Clone number: HL344
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

Associated diseases and disorders

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

Product promise

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9 product images

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296) at 1/5000 dilution
Lane 1: 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg
Lane 2: SARS-CoV NP transfected 293T whole cell extracts at 30 µg
Lane 3: SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts at 30 µg
Lane 4: MERS-CoV NP transfected 293T whole cell extracts at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
Paraffin embedded SARS-CoV-2 (COVID-19) infected human placenta (left) and normal human placenta (right) tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281296 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
Paraffin embedded SARS-CoV-2 (COVID-19) infected human lung tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281296 in immunohistochemical analysis.
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
Mock and transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281296 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (A and B) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with ab281296 at 1 μg/mL. HRP-conjugated Rabbit IgG antibody was diluted at 1/10000 and used to detect the primary antibody.
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281296 as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281296 (green) at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate bufferat pH 6.0 for 15 minutes.
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281296 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 0.074 nM
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296) at 1/5000 dilution
Lane 1: 293T whole cell extracts at 30 µg
Lane 2: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T whole cell extracts at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody