Mouse Monoclonal NCAP antibody. Carrier free. Suitable for IHC-P, ICC, WB, sELISA and reacts with SARS-CoV-2 samples. Cited in 2 publications.
Images
![Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--immunocytochemistry-img38032.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300)")
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--western-blot-img39077.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41866.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300)")
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--western-blot-img49705.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300)")
![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--sandwich-elisa-img133704.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (AB281300)")
Publications
Filter by
- Microbiology spectrum 11:e00503232023Exosome-Based Multivalent Vaccine: Achieving Potent Immunization, Broadened Reactivity, and Strong T-Cell Responses with Nanograms of Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMafalda Cacciottolo,Justin B Nice,Yujia Li,Michael J LeClaire,Ryan Twaddle,Ciana L Mora,Stephanie Y Adachi,Esther R Chin,Meredith Young,Jenna Angeles,Kristi Elliott,Minghao SunPubMed 37093009
- Frontiers in immunology 14:11202982023Exploring SARS-CoV-2 and coinfection in human erythrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana López-Farfán,Nerea Irigoyen,Elena Gómez-DíazPubMed 36993979
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | ICC | WB | sELISA | |
|---|---|---|---|---|
| SARS-CoV-2 | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV-2 | Dilution info 1 µg/mL | Notes - |
Target data
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661). May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414). May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
Alternative names
Nucleoprotein, N, Nucleocapsid protein, NC, Protein N
Recommended products
Mouse Monoclonal NCAP antibody. Carrier free. Suitable for IHC-P, ICC, WB, sELISA and reacts with SARS-CoV-2 samples. Cited in 2 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL455-MS
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.
- Biological function summary
The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.
- Pathways
The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.
- Associated diseases and disorders
The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
![Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--immunocytochemistry-img38032.jpg "Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
Mock and SARS-CoV-2 nucleocapsid transfected HEK 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 nucleocapsid (green) was labeled using ab281300 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--western-blot-img39077.jpg "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
Upper panel probed with ab281300.
All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution
Lane 1: 293T whole cell extracts (30 μg)
Lane 2: 293T whole cell extracts (30 μg) transfected with SARS-CoV NP
Lane 3: 293T whole cell extracts (30 μg) transfected with SARS-CoV-2 (COVID-19) NP
Lane 4: 293T whole cell extracts (30 μg) transfected with MERS-CoV NP
SecondaryAll lanes: HRP-conjugated anti-mouse IgG antibody
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41866.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
Mock and SARS-CoV-2 Nucleocapsid transfected HEK-293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281300 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval achieved with Citrate buffer at pH 6.0 for 15 minutes.
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--western-blot-img49705.jpg "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution
All lanes: Vero E6 whole cell extracts (30 μg)
SecondaryLane 1: HRP-conjugated anti-mouse IgG antibody
Lane 2: HRP-conjugated anti-mouse IgG antibody transfected with SARS-CoV-2 nucleocapsid
![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--sandwich-elisa-img133704.jpg "Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free ab281301 as capture antibody at concentration of 5 μg/mL and ab281300 as detection antibody at concentration of 1 μg/mL. HRP-Mouse IgG antibody at 1/10000 dilution wasused to detect the primary antibody. Sensitivity: 2.68 pg/mL.
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--western-blot-img43671.jpg "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
All lanes: Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution
Lane 1: 293T whole cell extracts (30 μg)
Lane 2: 293T whole cell extracts (30 μg) transfected with SARS-CoV-2 nucleocapsid
SecondaryAll lanes: HRP-conjugated anti-mouse IgG antibody
![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--sandwich-elisa-img85950.jpg "Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
Sandwich ELISA detection of non-transfected and SARS-CoV-2 , MERS-CoV and SARS-CoV Nucleocapsid transfected HEK-293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using 281301 as capture antibody at concentration of 5 μg/mL and ab281300 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--western-blot-img43671.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300), expandable thumbnail](https://content.abcam.com/products/images/sars-cov-2-nucleocapsid-protein-antibody-hl455-ms-bsa-and-azide-free-ab281300--sandwich-elisa-img85950.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)")
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