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AB284040

Anti-SARS-CoV-2 orf3d antibody [EPR25045-51]

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Rabbit Recombinant Monoclonal ORF3D antibody. Suitable for Flow Cyt (Intra), ICC/IF, I-ELISA, WB and reacts with Transfected cell line - SARS-CoV-2, Synthetic peptide - SARS-CoV-2, Recombinant fragment - SARS-CoV-2 samples.

View Alternative Names

Putative ORF3d protein

4 Images
Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)

Intracellular Flow Cytometry analysis of HEK-293T (Human embryonic kidney epithelial cell) cells transfected with a SARS-CoV-2 orf8 (Left) expression vector containing a myc tag or SARS-CoV2 orf3d (Right) expression vector containing GFP tag labeling SARS-CoV-2 orf3d with ab284040 at 1/500 dilution (Left and Right). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 647, ab150083) secondary antibody was used at 1/5000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling SARS-CoV-2 orf3d with primary antibody anti-SARS-CoV-2 orf3d (ab284040) at 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor® 594) (ab150089) (green) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a SARS-CoV2 orf3d expression vector containing a myc tag, no staining in HEK-293T cells transfected with a SARS-CoV2 orf8 expression vector containing a myc tag. Myc-Tag Mouse mab (Alexa Fluor® 647) (red) was used to counterstain cells at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Indirect ELISA - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)

Indirect ELISA showing primary antibody ab284040 binding to SARS-CoV2 ORF3d peptide (aa 12-32) . Plates were coated with SARS-CoV2 ORF3d peptide (aa 12-32) and recombinant SARS-CoV2 ORF8 (aa 16-121) protein (His tag) at 1000 ng/ml. Binding of ab284040 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

Western blot - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (AB284040)

ab284040 was shown to bind specifically to SARS-CoV2 Orf3d peptide in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Blocking buffer : 5% BSA in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] (ab284040) at 1/1000 dilution

Lane 1:

Orf3d peptide (aa12-32) at 0.5 µg

Lane 2:

Orf3d peptide (aa12-32) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/20000 dilution

Observed band size: 6 kDa

false

  • Carrier free

    Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25045-51

Isotype

IgG

Carrier free

No

Reacts with

SARS-CoV-2

Applications

Flow Cyt (Intra), WB, I-ELISA, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - SARS-CoV-2": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "SARS-CoV-2": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide - SARS-CoV-2": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "0.00002-1 µg/mL", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 orf3d protein also referred to as "Orf3d" in literature serves a unique function in the virus's life cycle. This viral protein has a mass of approximately 12 kDa and is expressed mainly in the cytoplasm of the infected host cells. The Orf3d protein contributes to the virus's ability to manipulate host cellular mechanisms although its detailed mechanical roles are not yet fully understood. Researchers have recently identified it as an open reading frame within the SARS-CoV-2 genome highlighting its importance in viral function.
Biological function summary

The SARS-CoV-2 orf3d protein affects the virus's replication and interaction with host defenses. While not part of any known larger protein complex it has shown effects in viral pathogenicity aiding the virus in evading host immune responses. The protein's activity potentially disrupts normal cellular processes within infected cells assisting the virus in maintaining an environment favorable for its replication.

Pathways

SARS-CoV-2 orf3d plays a part in both the viral replication and immune evasion pathways. It interacts with elements of host cell immune signaling to promote viral survival and replication. The protein is associated with pathways involving the virus's replication machinery potentially interacting with other proteins such as those encoded by SARS-CoV-2 like orf3a which similarly assist in viral infection processes.

SARS-CoV-2 orf3d has implications in COVID-19 pathogenesis. It may contribute to the severity and progression of symptoms observed in infected individuals. Its role in regulating immune response mechanisms shows connections to other viral proteins such as the SARS-CoV-2 nucleocapsid protein which also have implications in viral infection severity. Understanding Orf3d’s interactions could provide insights valuable for therapeutic developments targeting COVID-19.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ORF3D_SARS2

Additional targets

SARS-CoV-2 orf3d
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com