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AB313933

Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free

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(1 Publication)

Rabbit Monoclonal NS7B antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within SARS-CoV-2 7b conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ORF7b protein, ORF7b, Accessory protein 7b, 7b

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (AB313933)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (AB313933)

Immunohistochemical analysis of paraffin-embedded mock and SARS-CoV-2 ORF7b transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell pellets labeling SARS-CoV-2 (COVID-19) ORF7b with ab313933 at 1/1000 dilution (green). Blue : Fluoroshield with DAPI. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (AB313933)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (AB313933)

Immunofluorescent analysis of mock and SARS-CoV-2 (COVID-19) ORF7b transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 min labeling SARS-CoV-2 (COVID-19) ORF7b protein with ab313933 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (AB313933)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (AB313933)

All lanes:

Western blot - Anti-SARS-CoV-2 ORF7b antibody [HL1453] - BSA and Azide free (ab313933) at 1/2000 dilution

Lane 1:

Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

DDDDK-tag tagged SARS-CoV-2 (COVID19) ORF7b transfected HEK293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1453

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within SARS-CoV-2 7b conjugated to Keyhole Limpet Haemocyanin.

P0DTD8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 ORF7b protein also referred to as Open Reading Frame 7b is a protein encoded by the SARS-CoV-2 virus. It has an approximate mass of 14 kilodaltons and is expressed within the infected host cells. The ORF7b protein is expressed in the cytoplasm and is known to influence the replication or assembly of the virus. Researchers continue to study its precise role in the viral life cycle but it is understood to be integral to the virus's ability to proliferate effectively in host cells.
Biological function summary

The function and impact of the ORF7b protein is of significant interest. It does not appear to participate in a specific multi-protein complex but might interact transiently with other viral or host proteins to promote viral propagation. Its expression can influence the host cell's metabolic environment potentially modulating immune responses to favor viral survival and evade host defenses. This protein while small is regarded as a contributing factor to the host-cell interaction dynamics during viral infection.

Pathways

ORF7b protein is involved in manipulating host cell pathways to facilitate continued viral replication and assembly. Although direct interactions within major pathways are not well-defined ORF7b likely interacts with cellular immune signaling pathways which can alter the balance of immune responses. This protein may have indirect relationships with other viral proteins such as ORF7a impacting the virus's pathogenic profile and immune evasion strategies.

Studies suggest the ORF7b protein may be linked to the severity of COVID-19. Its activity could be contributing to lung tissue inflammation seen in severe cases of the disease potentially through interactions with host proteins involved in inflammatory responses. It may also have indirect implications in disease progression noting the general harm SARS-CoV-2 inflicts on lung tissue and possibly other organs. Understanding ORF7b's role could enhance insights into the mechanisms underlying COVID-19 pathology and inform therapeutic approaches targeting viral or immune regulatory processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 7b
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406211 PubMed39301916

2024

Lung-Targeted Lipid Nanoparticle-Delivered siUSP33 Attenuates SARS-CoV-2 Replication and Virulence by Promoting Envelope Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzheng Zhou,Yujie Liao,Lujie Fan,Xiafei Wei,Qiang Huang,Chuwei Yang,Wei Feng,Yezi Wu,Xiang Gao,Xiaotong Shen,Jian Zhou,Zanxian Xia,Zheng Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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