JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB284049

Anti-SARS-CoV-2 Protein 9b antibody [EPR25043-28] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SARS CoV-2 ORF9b protein antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Recombinant fragment - SARS-CoV-2 samples.

View Alternative Names

ORF9b protein, ORF9b, Accessory protein 9b, ORF-9b, Protein 9b, 9b

2 Images
Indirect ELISA - Anti-SARS-CoV-2 Protein 9b antibody [EPR25043-28] - BSA and Azide free (AB284049)
  • I-ELISA

Lab

Indirect ELISA - Anti-SARS-CoV-2 Protein 9b antibody [EPR25043-28] - BSA and Azide free (AB284049)

This data was developed using ab284035, the same antibody clone in a different buffer formulation.

Indirect ELISA- Anti-SARS-CoV2 ORF9b Protein Antibody (ab284035)

Indirect ELISA showing primary antibody ab284035 binding to recombinant SARS-CoV-2 ORF9b protein. Plates were coated with recombinant SARS-CoV-2 ORF9b protein (GST-tagged) and recombinant SARS-CoV-2 ORF3 protein (His-tagged) at 1000 ng/ml. Binding of ab284035 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

Western blot - Anti-SARS-CoV-2 Protein 9b antibody [EPR25043-28] - BSA and Azide free (AB284049)
  • WB

Lab

Western blot - Anti-SARS-CoV-2 Protein 9b antibody [EPR25043-28] - BSA and Azide free (AB284049)

This data was developed using ab284035, the same antibody clone in a different buffer formulation.

ab284035 was shown to bind specifically to SARS-CoV-2 ORF9b protein in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

ab92 is a Mouse monoclonal [3G10/1B3] to GST

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 Protein 9b antibody [EPR25043-28] (<a href='/en-us/products/primary-antibodies/sars-cov-2-protein-9b-antibody-epr25043-28-ab284035'>ab284035</a>) at 1/1000 dilution

All lanes:

Recombinant SARS-CoV2 ORF9b protein (GST-tagged) at 0.2 µg

Secondary

Lane 1:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lane 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Observed band size: 37 kDa

false

  • Unconjugated

    Anti-SARS-CoV-2 Protein 9b antibody [EPR25043-28]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25043-28

Isotype

IgG

Carrier free

Yes

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB283958

Anti-SARS-COV-2 nsp3 antibody [EPR24842-84]

20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size

ELISA - Anti-SARS-COV-2 nsp3 antibody [EPR24842-84] (AB283958)

  • 3
  • 3
Primary Antibodies

AB116

Anti-beta Galactosidase antibody [DC1 4C7]

Trial Size

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (AB116)

  • 0
  • 0
Primary Antibodies

AB303516

Anti-Hsp 90 alpha antibody [EPR27270-11]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp 90 alpha antibody [EPR27270-11] (AB303516)

  • 0
  • 0
Proteins & Peptides

AB277617

Recombinant human coronavirus SARS-CoV-2 RNA-dependent RNA polymerase (RDRP) protein (Active)

Functional Studies - Recombinant human coronavirus SARS-CoV-2 RNA-dependent RNA polymerase (RDRP) protein (Active) (AB277617)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "SARS-CoV-2": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
style
left-column

Product details

ab284049 is the carrier-free version of ab284935

Protein 9b and ORF9b are alternative names for Uniprot P0DTD2.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 ORF9b protein sometimes referred to as ORF9b protein is a small accessory protein with a mass of approximately 9 kDa. This protein is encoded by the open reading frame 9b (ORF9b) of the SARS-CoV-2 virus. It is expressed within the infected host cells where the virus replicates. ORF9b protein localizes to the mitochondria and plays a structural role in modulating host cellular processes during infection.
Biological function summary

The ORF9b protein interacts with the host immune system by inhibiting the type I interferon signaling pathway. This protein acts as a part of the viral evasion mechanisms allowing SARS-CoV-2 to escape host immune detection. ORF9b does not form part of a larger protein complex by itself but operates through interactions with host proteins affecting the host's antiviral responses.

Pathways

ORF9b disrupts the type I interferon immune signaling pathway one of the host's key antiviral defenses. It interacts with proteins such as Tom70 a mitochondrial import receptor. This interaction hampers the signaling cascade leading to reduced production of interferon which normally activates immune responses against viral infections. ORF9b's role in these pathways directly counteracts the host's ability to mount a timely defense against SARS-CoV-2 infection.

ORF9b is associated with the pathogenesis of COVID-19 the disease caused by SARS-CoV-2. Its ability to suppress immune signaling can contribute to the severity of COVID-19 by enabling viral persistence and exacerbating inflammatory responses. Additionally ORF9b interaction with ubiquitin system proteins such as MAVS facilitates immune evasion highlighting its role in the disease progression and severity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in inhibiting the host innate immune response by targeting the mitochondrial-associated innate immune response. Acts by binding to host TOMM70, inhibiting its binding to HSP90AB1 thereby disrupting the interferon activation pathway.
See full target information 9b

Additional targets

SARS CoV-2 ORF9b protein
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com