Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (AB272504)

Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung but not in non-COVID-19 patient lung.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (AB272504)

Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung.

• IHC-P

AbReview75222****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (AB272504)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of Covid-19 positive lung, autopsy sample tissue. Stained with ab272504 at 1/200 dilution. Blocking was done with Nanostring Buffer W for 1 hour at 25°C. The sample was incubated with the primary antibody and Nanostring Buffer W at 12µg/ml for 12 hours at 4°C. Antigen retrieval method was heat mediated, Citrate Buffer pH 6.0.

This image is courtesy of an anonymous Abreview

• IHC-P

AbReview76522****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (AB272504)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human Skin (Covid-19 positive, autopsy sample) tissue. Stained with ab272504 at 1/200 dilution. Secondary antibody used was HRP Novolink Max Polymer Detection System. Blocking was done with 10% milk for 30 minutes at 21°C. The sample was incubated with the primary antibody and 1% BSA for 12 hours at 4°C. Antigen retrieval method was heat mediated, Dako Retrieval Solution (S1699).

This image is courtesy of an anonymous Abreview

• ELISA

Supplier Data

ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (AB272504)

A direct ELISA was performed using antigen or control peptide as coating antigen and ab272504 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 0.5 ng/ml to 1000 ng/ml.

• WB

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Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (AB272504)

Primary incubation for 1 hour at room temperature.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) at 1 µg/mL

Lane 1:

Wild-type HEK-293 cell lysate at 15 µg

Lane 2:

HEK-293 overexpressing SARS-CoV-2 spike glycoprotein, cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

false