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Mouse Recombinant Monoclonal SPIKE antibody. Suitable for WB, I-ELISA and reacts with SARS-CoV-2 samples.

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Images

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (AB322272), expandable thumbnail
  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (AB322272), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBI-ELISA
SARS-CoV-2
Tested
Tested

Tested
Tested

Species
SARS-CoV-2
Dilution info
1/500
Notes

-

Tested
Tested

Species
SARS-CoV-2
Dilution info
-
Notes

-

Target data

Mouse Recombinant Monoclonal SPIKE antibody. Suitable for WB, I-ELISA and reacts with SARS-CoV-2 samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
8B12-C2
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

This human monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free ab282749). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (ab322272), expandable thumbnail

    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (ab322272)

    Indirect ELISA showing primary antibody ab322272 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105) and Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) at 1000 ng/ml. Binding of ab322272 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (Goat Anti-Human IgG Fc (HRP) preadsorbed ab98624) was used at 1:5000.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (ab322272), expandable thumbnail

    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (ab322272)

    False colour image of Western blot: Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Human IgG1 (Chimeric) (ab322272) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (Anti-6X His tag® antibody [HIS.H8] ab18184) loading control staining at 1/200 dilution, shown in red. In Western blot, ab322272 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (Goat Anti-Human IgG Fc (HRP) preadsorbed ab98624) at 1:10000 and Goat anti-Mouse IgG H&L 680RD at 1:20000.

    All lanes: Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (ab322272) at 1/500 dilution

    Lane 1: Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.2 µg

    Lane 2: SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

    Lane 3: SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (Goat Anti-Human IgG Fc (HRP) preadsorbed ab98624) at 1/10000 dilution

    Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 120-160 kDa

    Exposure time: 5s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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