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Human Recombinant Monoclonal SPIKE antibody. Suitable for ELISA, I-ELISA and reacts with Recombinant full length protein - SARS-CoV-2, Recombinant full length protein - SARS-CoV, Recombinant fragment - SARS-CoV-2 samples. Cited in 27 publications. Immunogen corresponding to Cell preparation containing SARS-CoV-2 Spike Glycoprotein S1 protein.
Alternative names=Spike glycoprotein, S glycoprotein, E2, Peplomer protein, 2, S
- Isotype
IgG1
- Host species
Human
- Storage buffer
Preservative: 0.02% Proclin 300
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Cell preparation containing SARS-CoV-2 Spike Glycoprotein S1 protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | I-ELISA | |
|---|---|---|
| Recombinant fragment - SARS-CoV-2 | Not recommended | Tested |
| Recombinant full length protein - SARS-CoV | Tested | Not recommended |
| Recombinant full length protein - SARS-CoV-2 | Tested | Not recommended |
| SARS-CoV | Predicted | Predicted |
| SARS-CoV-2 | Predicted | Predicted |
Images
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - SARS-CoV-2, Recombinant full length protein - SARS-CoV | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV, SARS-CoV-2 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - SARS-CoV-2 | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - SARS-CoV-2 | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species SARS-CoV, SARS-CoV-2 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - SARS-CoV-2, Recombinant full length protein - SARS-CoV | Dilution info - | Notes - |
Recommended products
Human Recombinant Monoclonal SPIKE antibody. Suitable for ELISA, I-ELISA and reacts with Recombinant full length protein - SARS-CoV-2, Recombinant full length protein - SARS-CoV, Recombinant fragment - SARS-CoV-2 samples. Cited in 27 publications. Immunogen corresponding to Cell preparation containing SARS-CoV-2 Spike Glycoprotein S1 protein.
Alternative names=Spike glycoprotein, S glycoprotein, E2, Peplomer protein, 2, S
Key facts
- Isotype
IgG1
- Host species
Human
- Storage buffer
Preservative: 0.02% Proclin 300
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Cell preparation containing SARS-CoV-2 Spike Glycoprotein S1 protein. The exact immunogen used to generate this antibody is proprietary information.
- Clone number
CR3022
- Purification technique
Affinity purification Protein A
- Epitope
This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. 2020).
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
The original CR3022 antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV. This antibody was shown to neutralize SARS-COV in a concerted action with clone CR3014. Presence of both antibodies delivers a blocking action of the SARS-COV RBD-ACE2 interaction, by binding two distinct and functional epitopes (16796401).
CR3022 has been shown to bind with a high affinity to SARS-CoV2 (32416259, 32413276). Structural modelling has confirmed that CR3022 targets a conserved epitope between SARS-CoV and SARS-CoV2 in the RBD domain (32245784, 32065055). Precisely, this antibody binds to the 'open' conformation of the spike protein to the amino acids 318-510 in the S1 domain of the SARS-CoV as well as SARS-CoV-2 strains (32245784, Joyce et al. 2020). The antibody is also able to bind the P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope of clone CR3022 does not overlap with the ACE2 binding site of SARS-COV2 (32065055). Therefore whilst CR3022 can neutralise SARS-COV in in a concerted action with clone CR3014, CR3022 is not believed to independently neutralise SARS-COV2, based on in vitro studies (32226289, 32065055, 32383254, 32416259).
Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab273073) is a Human IgG1 Recombinant version of CR3022 for research use only. CR3022 is also available as a chimeric rabbit antibody (ab273074).
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
ab273073 was developed to have a human IgG1 isotype.
Other isotypes of clone CR3022 available:
ab278112 – human IgA
ab278111 – human IgM
ab273074 – rabbit IgG
ab273886 – rat IgG2a
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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