Mouse Recombinant Monoclonal SPIKE antibody. Suitable for I-ELISA and reacts with Recombinant fragment - SARS-CoV-2 samples.
IgG1
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
I-ELISA | |
---|---|
Recombinant fragment - SARS-CoV-2 | Tested |
SARS-CoV-2 | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - SARS-CoV-2 | Dilution info 0.016-1000 ng/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species SARS-CoV-2 | Dilution info - | Notes - |
Mouse Recombinant Monoclonal SPIKE antibody. Suitable for I-ELISA and reacts with Recombinant fragment - SARS-CoV-2 samples.
IgG1
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR24852-116
Affinity purification Protein A
Our data (not shown) suggests this RabMAb is interacting with the non-receptor binding domain of SARS-CoV-2 Spike Glycoprotein S1 with a higher affinity (~ 71x ) than the receptor binding domain of SARS-CoV-2 Spike Glycoprotein S1.
This antibody cross-reacts with the SARS-CoV-2 Omicron variant spike protein.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] ab283942). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 Spike Glycoprotein S1 also known as the G10 spike or glycoprotein spike plays an important role in allowing the virus to attach and enter host cells. This protein with a mass of approximately 180 kDa is located on the surface of the virus and forms the outer spikes observed in coronaviruses. Expression of the spike glycoprotein is in virus-infected cells where it facilitates the interaction with host cell receptors. The S1 subunit includes a receptor-binding domain that specifically binds to the human angiotensin-converting enzyme 2 (ACE2) receptors initiating the infection process.
The spike glycoprotein S1 mediates the fusion of the viral and cellular membranes which is necessary for viral entry. It forms part of a larger trimeric complex comprising S1 and S2 subunits. This complex undergoes conformational changes that drive the membrane fusion process. The glycoprotein contains multiple glycosylation sites which help shield the virus from the host immune response. The proper function and presentation of this glycoprotein are critical for efficient viral spread and infection establishment.
The spike glycoprotein S1 is integral to the viral infection pathway and host immune evasion. It interacts with the renin-angiotensin system by binding to the ACE2 receptor disrupting normal receptor activity. This interaction not only facilitates viral entry but also impacts the homeostatic functions typically mediated by ACE2 which include blood pressure regulation. Additionally the spike protein is involved in downstream activation of immune signaling pathways including those related to inflammation and cytokine production which may involve proteins such as IL-6.
Infection with the spike glycoprotein S1 is directly related to COVID-19. The binding to ACE2 receptors is linked to the pathology of the disease contributing to respiratory symptoms and in severe cases acute respiratory distress syndrome (ARDS). Through the IL-6 signaling pathway the spike protein is indirectly connected to cytokine release syndrome often observed in severe COVID-19 cases. This connection highlights the importance of targeting this glycoprotein for potential therapeutic interventions and diagnostics such as ELISA SARS-CoV-2 tests and the development of anti-spike antibodies available on platforms like antispark.com for research and clinical purposes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Indirect ELISA- Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - Mouse IgG1 (Chimeric) (ab323455).
Indirect ELISA showing primary antibody ab323455 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) and Recombinant human coronavirus SARS spike glycoprotein (Tagged, Recombinant Human coronavirus SARS spike glycoprotein (Tagged) ab270844) at 1000 ng/ml. Binding of ab323455 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Mouse IgG Fc (HRP) preadsorbed (Goat Anti-Mouse IgG Fc (HRP) preadsorbed ab98717) was used at 1:5000.
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