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AB283947

Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free

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Rabbit Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples.

View Alternative Names

2, S, Spike glycoprotein, S glycoprotein, E2, Peplomer protein

4 Images
Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)
  • I-ELISA

Lab

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)

This data was developed using ab283946, the same antibody clone in a different buffer formulation.

Indirect ELISA showing primary antibody ab283946 binding to the antigen ab272105 (recombinant human coronavirus SARS-CoV-2 Spike glycoprotein S1 (Fc Chimera)). Plates were coated with recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera, ab272105) at 1000 ng/ml. Binding of ab283946 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)
  • I-ELISA

Lab

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)

This data was created using ab283946, the same clone with a different buffer formulation.

Indirect ELISA showing primary antibody ab283946 binding to recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera, ab272105), SARS-CoV-2 spike S1+S2 ECD-His recombinant protein and recombinant human coronavirus SARS-CoV-2 spike glycoprotein RBD (His tagged, ab275986) .

Plates were coated with recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera, ab272105), SARS-CoV-2 spike S1+S2 ECD-His recombinant protein, SARS-CoV spike S1+S2 ECD-His recombinant protein and recombinant human coronavirus SARS-CoV-2 spike glycoprotein RBD (His tagged, ab275986) at 1000 ng/ml. Binding of ab283946 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)
  • WB

Lab

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)

This data was developed using ab283946, the same antibody clone in a different buffer formulation.

Observed band size : 200 kDa

Calculated : 140kDa

Lane 1 & 2 : Red – loading control Goat anti-DDDDK tag antibody (ab95045, Binds to FLAG tag sequence) observed at 200 kDa

Lanes 3 & 4 : Green – ab283946 observed at 200 kDa

ab283946 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) and Donkey anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/20000 dilution.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-rbd-antibody-epr24852-174-ab283946'>ab283946</a>) at 1/500 dilution

Lanes 1 and 3:

Expi293 cells transfected with SARS-CoV2 3xFlag Spike Protein at 40 µg

Lanes 2 and 4:

Expi293 cells transfected with SARS-CoV1 3xFlag Spike Protein at 40 µg

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Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)
  • WB

Lab

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - BSA and Azide free (AB283947)

This data was developed using ab283946, the same antibody clone in a different buffer formulation.

Observed band size : 125 kDa

Lane 1 : Red – loading control Mouse anti-6x His tag antibody (ab18184) observed at 125 kDa

Lanes 2 : Green – ab283946 observed at 125 kDa

ab283946 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/20000 dilution.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-rbd-antibody-epr24852-174-ab283946'>ab283946</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a>) at 0.5 µg

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  • Unconjugated

    Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174]

  • Unconjugated

    Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] - Mouse IgG1 (Chimeric)

  • Carrier free

    Anti-SARS-CoV-2 S Glycoprotein S1 (RBD) antibody [EPR24852-174] -Ms IgG1 (Chimeric) BSA & Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24852-174

Isotype

IgG

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - SARS-CoV-2": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "SARS-CoV-2": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab283947 is the carrier free version of ab283946.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 Spike Glycoprotein S1 also known as the G10 spike or glycoprotein spike plays an important role in allowing the virus to attach and enter host cells. This protein with a mass of approximately 180 kDa is located on the surface of the virus and forms the outer spikes observed in coronaviruses. Expression of the spike glycoprotein is in virus-infected cells where it facilitates the interaction with host cell receptors. The S1 subunit includes a receptor-binding domain that specifically binds to the human angiotensin-converting enzyme 2 (ACE2) receptors initiating the infection process.
Biological function summary

The spike glycoprotein S1 mediates the fusion of the viral and cellular membranes which is necessary for viral entry. It forms part of a larger trimeric complex comprising S1 and S2 subunits. This complex undergoes conformational changes that drive the membrane fusion process. The glycoprotein contains multiple glycosylation sites which help shield the virus from the host immune response. The proper function and presentation of this glycoprotein are critical for efficient viral spread and infection establishment.

Pathways

The spike glycoprotein S1 is integral to the viral infection pathway and host immune evasion. It interacts with the renin-angiotensin system by binding to the ACE2 receptor disrupting normal receptor activity. This interaction not only facilitates viral entry but also impacts the homeostatic functions typically mediated by ACE2 which include blood pressure regulation. Additionally the spike protein is involved in downstream activation of immune signaling pathways including those related to inflammation and cytokine production which may involve proteins such as IL-6.

Infection with the spike glycoprotein S1 is directly related to COVID-19. The binding to ACE2 receptors is linked to the pathology of the disease contributing to respiratory symptoms and in severe cases acute respiratory distress syndrome (ARDS). Through the IL-6 signaling pathway the spike protein is indirectly connected to cytokine release syndrome often observed in severe COVID-19 cases. This connection highlights the importance of targeting this glycoprotein for potential therapeutic interventions and diagnostics such as ELISA SARS-CoV-2 tests and the development of anti-spike antibodies available on platforms like antispark.com for research and clinical purposes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Spike protein S1. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2 (PubMed : 32142651, PubMed : 32155444, PubMed : 33607086). When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane (PubMed : 34561887). When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis leading to fusion of the virion membrane with the host endosomal membrane (PubMed : 32075877, PubMed : 32221306). Alternatively, may use NRP1/NRP2 (PubMed : 33082294, PubMed : 33082293) and integrin as entry receptors (PubMed : 35150743). The use of NRP1/NRP2 receptors may explain the tropism of the virus in human olfactory epithelial cells, which express these molecules at high levels but ACE2 at low levels (PubMed : 33082293). The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed : 32817270).. Spike protein S2. Precursor of the fusion protein processed in the biosynthesis of the S protein and the formation of virus particle. Mediates fusion of the virion and cellular membranes by functioning as a class I viral fusion protein. Contains two viral fusion peptides that are unmasked after cleavage. The S2/S2' cleavage occurs during virus entry at the cell membrane by host TMPRSS2 (PubMed : 32142651) or during endocytosis by host CSTL (PubMed : 32703818, PubMed : 34159616). In either case, this triggers an extensive and irreversible conformational change leading to fusion of the viral envelope with the cellular cytoplasmic membrane, releasing viral genomic RNA into the host cell cytoplasm (PubMed : 34561887). Under the current model, the protein has at least three conformational states : pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During fusion of the viral and target cell membranes, the coiled coil regions (heptad repeats) adopt a trimer-of-hairpins structure and position the fusion peptide in close proximity to the C-terminal region of the ectodomain. Formation of this structure appears to promote apposition and subsequent fusion of viral and target cell membranes.. Spike protein S2'. Subunit of the fusion protein that is processed upon entry into the host cell. Mediates fusion of the virion and cellular membranes by functioning as a class I viral fusion protein. Contains a viral fusion peptide that is unmasked after S2 cleavage. This cleavage can occur at the cell membrane by host TMPRSS2 or during endocytosis by host CSTL (PubMed : 32703818, PubMed : 34159616). In either case, this triggers an extensive and irreversible conformational change that leads to fusion of the viral envelope with the cellular cytoplasmic membrane, releasing viral genomic RNA into the host cell cytoplasm (PubMed : 34561887). Under the current model, the protein has at least three conformational states : pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During fusion of the viral and target cell membranes, the coiled coil regions (heptad repeats) adopt a trimer-of-hairpins structure and position the fusion peptide in close proximity to the C-terminal region of the ectodomain. Formation of this structure appears to promote apposition and subsequent fusion of viral and target cell membranes.
See full target information S

Additional targets

S
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Product promise

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