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AB284651

Anti-SARS-CoV-2 Spike RBD antibody [CA11]

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Rabbit Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for Flow Cyt, ELISA, SPR and reacts with Transfected cell line - SARS-CoV-2, SARS-CoV-2 samples.
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Flow Cytometry - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)

Flow cytomery neutralizing assay of ab284651 (plotted graphs) : ACE2 transfected cells were incubated with biotinylated spike protein (0.5 ug/mL) in the presence or absence of antibody. Binding of spike protein was detected with DyLight488 conjugated StreptAvidin. ab284651 efficiently blocked the binding of wild type and UK variant (B.1.1.7) to ACE2 expressing HEK-293 cells.

Flow Cytometry - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)

Flow cytometry neutralizing assay : ab284651 displayed dose dependent blocking of spike proteins (wild type and UK variant) to ACE2 expressing cells.

ELISA - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)
  • ELISA

Supplier Data

ELISA - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)

Binding of ab284651 to SARS-CoV-2 variants (UK and South Africa) : ab284651 shows good cross reactivity to both variants B.1.1.7 (UK) and B.1.351 (S. Africa).

Surface Plasmon Resonance - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)
  • SPR

Supplier Data

Surface Plasmon Resonance - Anti-SARS-CoV-2 Spike RBD antibody [CA11] (AB284651)

Affinity assessment of ab284651 to wild type and B.1.1.7 (UK) S1+S2 trimers. ab284651 very low (pM) KD to both variants.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

CA11

Isotype

IgG

Light chain type

kappa

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ELISA, Flow Cyt, SPR

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "SPR" : {"fullname" : "Surface Plasmon Resonance", "shortname":"SPR"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.001-2 µg/mL", "ELISA-species-notes": "<p></p>", "SPR-species-checked": "testedAndGuaranteed", "SPR-species-dilution-info": "", "SPR-species-notes": "<p></p>" }, "Transfected cell line - SARS-CoV-2": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.01-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "SPR-species-checked": "notRecommended", "SPR-species-dilution-info": "", "SPR-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 Spike RBD also known commonly as the Receptor Binding Domain of the spike protein plays an important role in viral entry into host cells. This domain has a mass of approximately 21 kDa. It is located on the surface of the virus and facilitates binding to the host cell receptor primarily ACE2 which permits viral entry and replication. The Spike RBD is also a target for neutralizing antibodies which are essential in immune response against the virus. The domain displays various mutations particularly in variants of concern such as Omicron which can affect binding efficiency and immune evasion.
Biological function summary

The SARS-CoV-2 Spike RBD interacts directly with the host ACE2 receptor to mediate entry of the virus into host cells. This interaction is necessary for the virus to fuse with the host cell membrane which allows viral RNA to enter the host cell and begin replication. The Spike protein of which the RBD is a part forms a trimeric complex on the virus surface that is important for host interaction. Variations in the Spike RBD such as mutations like Arg319-Phe541 have significant impacts on the binding affinity to ACE2 and the effectiveness of vaccine-elicited antibodies.

Pathways

The Spike RBD of SARS-CoV-2 is vital in the entry pathway of the virus into the host cell. It is prominently involved in the ACE2-mediated signaling pathway with ACE2 playing the key role as the cellular receptor. This pathway is integral to the pathogenesis of COVID-19. Additionally the presence of the virus in host cells can trigger inflammatory pathways via infection-induced signaling cascades which can lead to exacerbated immune responses.

The SARS-CoV-2 Spike RBD is inherently linked to COVID-19 pathogenesis. Variants such as Omicron have alterations within the RBD that may confer increased transmissibility and resistance to neutralizing antibodies. These mutations can influence disease severity and vaccine effectiveness. The interaction between the Spike RBD and ACE2 receptor underlies the symptomatic manifestations of COVID-19 including respiratory distress and systemic inflammation. The emergence of RBD-targeted therapeutics and vaccines addresses its critical role in infection aiming to block the binding and prevent disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS-CoV-2 Spike RBD
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Product promise

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For full details, please see our Terms & Conditions

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