AB281304

Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for ICC/IF, FuncS (Neut/Block), sELISA, I-ELISA and reacts with SARS-CoV-2, Recombinant fragment - SARS-CoV-2 samples.

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Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike RBD stained with ab281304 at 1/600 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).

Functional Studies (Neut/Block) - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

FuncS (Neut/Block)

Supplier Data

Functional Studies (Neut/Block) - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) coated at 2 μg/mL binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag at 3000 ng/mL. ACE2 binding was inhibited by increasing concentrations of ab281304 (14-10000 ng/mL). Bound ACE2 protein was detected by HRP-Goat Anti-Mouse IgG antibody at 1/10000 dilution.

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

sELISA

Supplier Data

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA detection of non-transfected and SARS-CoV-2 (COVID-19) Spike, MERS-CoV Spike, or SARS-CoV Spike transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.
Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.

sELISA

Supplier Data

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugatedab281304 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.

sELISA

Supplier Data

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.

sELISA

Supplier Data

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.

Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

I-ELISA

Supplier Data

Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (4000-62.5 ng/mL). Coated protein was probed with ab281304 at 1 μg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution was used to detect bound primary antibody.

sELISA

Supplier Data

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using a SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at 5 μg/mL and ab281304 as detection antibody at concentration of 1 μg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution and used to detect the primary antibody.

I-ELISA

Supplier Data

Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with ab281304 (160-1.91*10^-5 nM). HRP-Rabbit IgG antibody at 1/10000 dilituion detected bound primary antibody.
EC50 : 25.13 pM

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1004

Isotype

IgG1

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, FuncS (Neut/Block), I-ELISA, sELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - SARS-CoV-2": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "testedAndGuaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "This antibody inhibits infection of mammalian cells by live SARS-CoV-2.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "SARS-CoV-2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 Spike RBD also known commonly as the Receptor Binding Domain of the spike protein plays an important role in viral entry into host cells. This domain has a mass of approximately 21 kDa. It is located on the surface of the virus and facilitates binding to the host cell receptor primarily ACE2 which permits viral entry and replication. The Spike RBD is also a target for neutralizing antibodies which are essential in immune response against the virus. The domain displays various mutations particularly in variants of concern such as Omicron which can affect binding efficiency and immune evasion.

Biological function summary

The SARS-CoV-2 Spike RBD interacts directly with the host ACE2 receptor to mediate entry of the virus into host cells. This interaction is necessary for the virus to fuse with the host cell membrane which allows viral RNA to enter the host cell and begin replication. The Spike protein of which the RBD is a part forms a trimeric complex on the virus surface that is important for host interaction. Variations in the Spike RBD such as mutations like Arg319-Phe541 have significant impacts on the binding affinity to ACE2 and the effectiveness of vaccine-elicited antibodies.

Pathways

The Spike RBD of SARS-CoV-2 is vital in the entry pathway of the virus into the host cell. It is prominently involved in the ACE2-mediated signaling pathway with ACE2 playing the key role as the cellular receptor. This pathway is integral to the pathogenesis of COVID-19. Additionally the presence of the virus in host cells can trigger inflammatory pathways via infection-induced signaling cascades which can lead to exacerbated immune responses.

The SARS-CoV-2 Spike RBD is inherently linked to COVID-19 pathogenesis. Variants such as Omicron have alterations within the RBD that may confer increased transmissibility and resistance to neutralizing antibodies. These mutations can influence disease severity and vaccine effectiveness. The interaction between the Spike RBD and ACE2 receptor underlies the symptomatic manifestations of COVID-19 including respiratory distress and systemic inflammation. The emergence of RBD-targeted therapeutics and vaccines addresses its critical role in infection aiming to block the binding and prevent disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SARS-CoV-2 Spike RBD

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Functional Studies (Neut/Block) - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1004] - BSA and Azide free (AB281304)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise