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AB320094

Anti-SARS-CoV2 envelope protein antibody [EPR24857-260]

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Rabbit Recombinant Monoclonal VEMP antibody. Suitable for WB and reacts with Transfected cell lysate - SARS-CoV-2 samples.

View Alternative Names

4, E, Envelope small membrane protein, sM protein

1 Images
Western blot - Anti-SARS-CoV2 envelope protein antibody [EPR24857-260] (AB320094)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV2 envelope protein antibody [EPR24857-260] (AB320094)

Western blot : Rabbit Monoclonal to SARS-CoV2 Envelope Protein (ab320094) staining at 1000 dilution, shown in Green; Mouse anti-GAPDH antibody [6C5] (ab8245) at 1/20000 dilution, shown in red.

In Western blot, ab320094 was shown to bind specifically to SARS-CoV2 Envelope protein. A band was observed at 8 kDa in HEK-293T overexpressing SARS-CoV2 Envelope cell lysates with no signal observed at this size in HEK-293T mock transfected cell lysate. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SARS-CoV2 envelope protein antibody [EPR24857-260] (ab320094) at 1/1000 dilution

Lane 1:

HEK-293T mock transfected cell lysate at 20 µg

Lane 2:

HEK-293T overexpressing SARS-CoV2 Envelope cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 8 kDa,37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24857-260

Isotype

IgG

Carrier free

No

Reacts with

SARS-CoV-2

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 envelope protein also known as E protein plays an important role in viral assembly and release. It is a small structural protein with a mass of approximately 8.4 kDa and is expressed on the surface of the coronavirus. E protein is an integral membrane protein that localizes to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) where it assists in the assembly of the virus envelope. It contains multiple transmembrane domains that enable it to interact with other viral components inside the host cell.
Biological function summary

The SARS-CoV-2 E protein facilitates the virus budding process and contributes to the pathogenicity of the virus. It is a component of the virus envelope which maintains the structural integrity of the virion. E protein is also involved in ion channel activity acting in the formation of viroporins that modulate host cell environments. This ion channel activity aids in virus-host cell interactions and can impact cell signaling pathways advancing the virus's pathology.

Pathways

The SARS-CoV-2 E protein engages in critical processes of viral assembly and pathogenesis. It plays roles in the viral replication pathway and is implicated in the formation of virus-like particles (VLPs). The E protein interacts with other viral proteins such as the membrane protein (M) and nucleocapsid protein (N) coordinating the assembly and packaging of the virion. These interactions are essential for the completion of the viral life cycle within the host cell influencing virus infectivity and stability.

SARS-CoV-2 E protein is directly associated with COVID-19 the disease caused by the coronavirus. The protein contributes to the virulence of the virus and can influence the clinical manifestations of COVID-19. Researchers have explored its links to acute respiratory distress syndrome (ARDS) as it can affect immune responses and inflammation in the lungs. The protein's interaction with the spike protein (S) is critical in understanding the disease's progression as these proteins collectively enable the virus to evade the host immune system and facilitate successful infection.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed : 33229438). May act by slowing down the cell secretory pathway (PubMed : 33229438). May interfere with tight-junction stability by interacting with host MPP5. This would result in disruption of epithelial barriers, thereby amplifying inflammatory processes (PubMed : 32891874).
See full target information E
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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