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AB273434

Anti-SARS Nucleocapsid Protein antibody [6H3]

0

(1 Review)

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(11 Publications)

Mouse Monoclonal NCAP antibody. Carrier free. Suitable for ICC/IF, IP, WB, sELISA and reacts with SARS-CoV-2, SARS-CoV, Recombinant full length protein - SARS-CoV-2 samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within SARS-CoV N aa 100 to C-terminus.

View Alternative Names

9a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

8 Images
Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • IP

Supplier Data

Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

SARS Nucleocapsid Protein was immunoprecipitated from 2μg of SARS-CoV2 Nucleocapsid Protein transfected HEK-293T whole cell lysate with either ab273434 (Lane 3) or a control IgG (Lane 2).

Western blot was performed from the immunoprecipitate using ab273434. An

HRP-conjugated anti mouse IgG antibody, was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

Predicted band size: 46 kDa

false

Sandwich ELISA - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 nucleocapsid protein using a different anti-SARS-CoV-2 capture antibody at concentration of 5 μg/mL and ab273434 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) was used at 1/10000 to detect the primary antibody.

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • WB

Supplier Data

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

10% SDS-PAGE

All lanes:

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T cells at 30 µg

Lane 2:

SARS-CoV NP transfected HEK-293T cells at 30 µg

Lane 3:

SARS-CoV2 (COVID-19) NP transfected HEK-293T cells

Lane 4:

MERS-CoV NP transfected HEK-293T cells at 30 µg

Predicted band size: 46 kDa

false

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • WB

Supplier Data

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

12% SDS-PAGE

All lanes:

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution

All lanes:

SARS-CoV-2 (COVID-19) nucleocapsid protein at 0.5 µg

Secondary

All lanes:

HRP-conjugated anti-Mouse IgG

Predicted band size: 46 kDa

false

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • WB

Supplier Data

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

Vero E6 cells were analysed at the indicated time-points post-infection with SARS-CoV.

All lanes:

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

Lane 1:

SARS-CoV infected Vero E6 cells post infection after 0 hours

Lane 2:

SARS-CoV infected Vero E6 cells post infection after 8 hours

Lane 3:

SARS-CoV infected Vero E6 cells post infection after 16 hours

Lane 4:

SARS-CoV infected Vero E6 cells post infection after 24 hours

Predicted band size: 46 kDa

false

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • WB

Supplier Data

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

10% SDS-PAGE

All lanes:

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T whole cell extract at 30 µg

Lane 2:

Transfected HEK-293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-Mouse IgG

Predicted band size: 46 kDa

false

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • WB

Supplier Data

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

Cell lysates were analysed by WB 48 hours post-infection with SARS-CoV or SARS Cov-2 at a MOI of 0.01.

Lanes 1 - 2:

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/1000 dilution

Lane 3:

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

Lane 1:

Non-infected Caco-2 whole cell extract

Lane 2:

SARS-CoV infected Caco-2 whole cell extract

Lane 3:

SARS-CoV-2 infected Caco-2 whole cell extract

Predicted band size: 46 kDa

false

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)
  • WB

Supplier Data

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (AB273434)

12% SDS-PAGE

All lanes:

Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T whole cell extract at 30 µg

Lane 2:

SARS-CoV nucleocapsid-transfected (a partial fragment) HEK-293T whole cell extracts at 30 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6H3

Isotype

IgG1

Carrier free

Yes

Reacts with

SARS-CoV, SARS-CoV-2

Applications

sELISA, IP, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within SARS-CoV N aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P59595

Specificity

No cross-reactivity with MERS-CoV nucleocapsid protein is expected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "MERS-CoV": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - SARS-CoV-2": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "<p></p>" }, "SARS-CoV": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "SARS-CoV-2": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }
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Product details

Applications overview

Tick: Tested and Guaranteed to work X: Will not work —: No data

<img src="https://www.abcam.com/ps/Products/273/ab273434/Images/ab273434-394505-ab273434-applications-overview.jpg" height="125" width="675">

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.

Pathways

Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.

The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed : 17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed : 18055455).
See full target information N
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e70001 PubMed39558820

2024

Extracellular vesicles containing SARS-CoV-2 proteins are associated with multi-organ dysfunction and worse outcomes in patients with severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Diego de Miguel-Perez,Marisol Arroyo-Hernandez,Sabrina La Salvia,Muthukumar Gunasekaran,Edward M Pickering,Stephanie Avila,Etse Gebru,Eduardo Becerril-Vargas,Sergio Monraz-Perez,Kapil Saharia,Alison Grazioli,Michael T McCurdy,Matthew Frieman,Lisa Miorin,Alessandro Russo,Andrés F Cardona,Adolfo García-Sastre,Sunjay Kaushal,Fred R Hirsch,Djordje Atanackovic,Susmita Sahoo,Oscar Arrieta,Christian Rolfo

Cell reports. Medicine 4:101079 PubMed37327781

2023

Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Joann Diray-Arce,Slim Fourati,Naresh Doni Jayavelu,Ravi Patel,Cole Maguire,Ana C Chang,Ravi Dandekar,Jingjing Qi,Brian H Lee,Patrick van Zalm,Andrew Schroeder,Ernie Chen,Anna Konstorum,Anderson Brito,Jeremy P Gygi,Alvin Kho,Jing Chen,Shrikant Pawar,Ana Silvia Gonzalez-Reiche,Annmarie Hoch,Carly E Milliren,James A Overton,Kerstin Westendorf,Charles B Cairns,Nadine Rouphael,Steven E Bosinger,Seunghee Kim-Schulze,Florian Krammer,Lindsey Rosen,Nathan D Grubaugh,Harm van Bakel,Michael Wilson,Jayant Rajan,Hanno Steen,Walter Eckalbar,Chris Cotsapas,Charles R Langelier,Ofer Levy,Matthew C Altman,Holden Maecker,Ruth R Montgomery,Elias K Haddad,Rafick P Sekaly,Denise Esserman,Al Ozonoff,Patrice M Becker,Alison D Augustine,Leying Guan,Bjoern Peters,Steven H Kleinstein

Proceedings of the National Academy of Sciences of the United States of America 119:e2200592119 PubMed35858386

2022

Omicron mutations enhance infectivity and reduce antibody neutralization of SARS-CoV-2 virus-like particles.

Applications

Unspecified application

Species

Unspecified reactive species

Abdullah M Syed,Alison Ciling,Taha Y Taha,Irene P Chen,Mir M Khalid,Bharath Sreekumar,Pei-Yi Chen,G Renuka Kumar,Rahul Suryawanshi,Ines Silva,Bilal Milbes,Noah Kojima,Victoria Hess,Maria Shacreaw,Lauren Lopez,Matthew Brobeck,Fred Turner,Lee Spraggon,Takako Tabata,Melanie Ott,Jennifer A Doudna

Journal of virology 96:e0000222 PubMed35107372

2022

Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuo Ariumi

Veterinary pathology 59:602-612 PubMed35094625

2022

Comparative pathology of the nasal epithelium in K18-hACE2 Tg mice, hACE2 Tg mice, and hamsters infected with SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Pin Yu,Wei Deng,Linlin Bao,Yajin Qu,Yanfeng Xu,Wenjie Zhao,Yunlin Han,Chuan Qin

Cellular & molecular immunology 19:210-221 PubMed34983944

2022

SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenfeng Wang,Jiadi Lv,Pin Yu,Yajin Qu,Yabo Zhou,Li Zhou,Qiangqiang Zhu,Shunshun Li,Jiangping Song,Wei Deng,Ran Gao,Yuying Liu,Jiangning Liu,Wei-Min Tong,Chuan Qin,Bo Huang

Cell 184:6022-6036.e18 PubMed34838159

2021

Identification of a therapeutic interfering particle-A single-dose SARS-CoV-2 antiviral intervention with a high barrier to resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Sonali Chaturvedi,Gustavo Vasen,Michael Pablo,Xinyue Chen,Nathan Beutler,Arjun Kumar,Elizabeth Tanner,Sylvia Illouz,Donna Rahgoshay,John Burnett,Leo Holguin,Pei-Yi Chen,Blaise Ndjamen,Melanie Ott,Robert Rodick,Thomas Rogers,Davey M Smith,Leor S Weinberger

Clinical journal of the American Society of Nephrology : CJASN 16:1755-1765 PubMed34127485

2021

Evidence For and Against Direct Kidney Infection by SARS-CoV-2 in Patients with COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Luise Hassler,Fabiola Reyes,Matthew A Sparks,Paul Welling,Daniel Batlle

Nature communications 12:2506 PubMed33947851

2021

Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Diao,Chenhui Wang,Rongshuai Wang,Zeqing Feng,Ji Zhang,Han Yang,Yingjun Tan,Huiming Wang,Changsong Wang,Liang Liu,Ying Liu,Yueping Liu,Gang Wang,Zilin Yuan,Xiaotao Hou,Liang Ren,Yuzhang Wu,Yongwen Chen

Frontiers in immunology 12:661052 PubMed33995382

2021

SARS-CoV-2 Induces Lymphocytopenia by Promoting Inflammation and Decimates Secondary Lymphoid Organs.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Xiang,Zeqing Feng,Bo Diao,Chao Tu,Qinghua Qiao,Han Yang,Yi Zhang,Gang Wang,Huiming Wang,Chenhui Wang,Liang Liu,Changsong Wang,Longding Liu,Rong Chen,Yuzhang Wu,Yongwen Chen
View all publications
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