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AB275984

Anti-SARS Nucleocapsid Protein antibody [CR3009 (03-009)]

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Rabbit Recombinant Monoclonal NCAP antibody. Suitable for ELISA and reacts with SARS-CoV-2, SARS-CoV samples.

View Alternative Names

9a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

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ELISA - Anti-SARS Nucleocapsid Protein antibody [CR3009 (03-009)] (AB275984)
  • ELISA

Supplier Data

ELISA - Anti-SARS Nucleocapsid Protein antibody [CR3009 (03-009)] (AB275984)

ELISA Plate coated with SARS-CoV-2 Nucleocapsid Proteins at a concentration of 5 μg/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using ab275984. For detection, a 1/4000 dilution of HRP-labelled anti-rabbit antibody was used. Rabbit anti-Fluorescein antibody was used as a control.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

CR3009 (03-009)

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

SARS-CoV, SARS-CoV-2

Applications

ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "10 µg/mL", "ELISA-species-notes": "<p>A 3-fold serial dilution from 10,000 ng/ml.</p>" }, "SARS-CoV-2": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "10 µg/mL", "ELISA-species-notes": "<p>A 3-fold serial dilution from 10,000 ng/ml.</p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: 99.98% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.

Pathways

Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.

The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed : 17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed : 18055455).
See full target information N

Additional targets

SARS Nucleocapsid Protein
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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