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AB273436

Anti-SARS spike glycoprotein S1 antibody [7G12]

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Mouse Monoclonal SPIKE antibody. Suitable for IP, WB and reacts with Transfected cell lysate - Human, SARS-CoV samples.
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Immunoprecipitation - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)
  • IP

Supplier Data

Immunoprecipitation - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)

Western blot / immunoprecipitation analysis.

All lanes:

Immunoprecipitation - Anti-SARS spike glycoprotein S1 antibody [7G12] (ab273436) at 1/500 dilution

Lane 1:

Wild-type S form plasmid transfected into HEK-293T, whole cell lysate

Lane 2:

Mutated S (N1056K) plasmid transfected into HEK-293T, whole cell lysate

Lane 3:

Mutated S (D1128A) plasmid transfected into HEK-293T, whole cell lysate

Lane 4:

Mutated S (N1056K, D1128A) plasmid transfected into HEK-293T, whole cell lysate

Lane 5:

Mock transfected HEK-293T, whole cell lysate

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Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)
  • WB

Supplier Data

Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)

All lanes:

Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (ab273436) at 1/500 dilution

Lane 1:

Wild-type S form plasmid transfected into HEK-293T, whole cell lysate

Lane 2:

Mutated S (N1056K) plasmid transfected into HEK-293T, whole cell lysate

Lane 3:

Mutated S (D1128A) plasmid transfected into HEK-293T, whole cell lysate

Lane 4:

Mutated S (N1056K, D1128A) plasmid transfected into HEK-293T, whole cell lysate

Lane 5:

Mock transfected i HEK-293T, whole cell lysate

Predicted band size: 139 kDa

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Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)
  • WB

Supplier Data

Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)

5% SDS-PAGE

All lanes:

Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (ab273436) at 1/1000 dilution

Lane 1:

Non-transfected (-) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

SARS-CoV SΔ3-transfected (+) HEK-293T whole cell lysate at 30 µg

Predicted band size: 139 kDa

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Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)
  • WB

Lab

Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (AB273436)

Lanes 1 - 3 : Green - ab273436 observed at 200kDa.

ab273436 was shown to react with SARS spike glycoprotein S1 in western blot. Membranes were blocked in 3% milk before incubation with ab273436 overnight at 4°C at 1 μg/ml. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SARS spike glycoprotein S1 antibody [7G12] (ab273436) at 1 µg/mL

Lane 1:

SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate at 10 µg

Lane 2:

SARS-Cov2 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate at 10 µg

Lane 3:

MERS Spike protein (DDDDK-tagged) transfected HEK-293 cell lysate at 10 µg

Predicted band size: 139 kDa

Observed band size: 200 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7G12

Isotype

IgG1

Carrier free

No

Reacts with

SARS-CoV

Applications

WB, IP

applications

Specificity

This antibody detects SARS-CoV spike protein (S1 subunit), and may have minor cross-reactivity with SARS-CoV-2 spike protein. No cross-reactivity with MERS-CoV spike protein is expected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Applications overview

Tick: Tested and Guaranteed to work X: Will not work —: No data

<img src="https://www.abcam.com/ps/Products/273/ab273436/Images/ab273436-394497-applications-overview.jpg" height="129" width="677">

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS spike glycoprotein S1 also known as Spike S1 is a structural protein of the SARS coronavirus. This glycoprotein serves as the major determinant of host specificity and immune evasion mediating virus entry into host cells. Its function involves binding to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells. The Spike S1 component has an approximate molecular mass of 90 kDa and expresses heavily on the viral surface facilitating the attachment of the virus to the host cell membrane.
Biological function summary

The SARS spike glycoprotein S1 plays an important role in the viral entry process. It is part of the larger spike protein complex which consists of S1 and S2 subunits. The S1 subunit binds directly to the host ACE2 receptor initiating conformational changes that allow the S2 subunit to promote membrane fusion. This protein complex is critical for the fusion of the viral and cellular membranes a necessary step for viral entry and infection.

Pathways

The interaction of the S1 glycoprotein with the host ACE2 receptor activates the renin-angiotensin system pathway. This protein-receptor binding influences the downregulation of ACE2 and contributes to the pathogenesis of SARS. Additionally the Spike S1 protein influences inflammatory responses through its interaction with Toll-like receptors. This interaction triggers immune responses impacting related proteins such as pro-inflammatory cytokines and chemokines.

The SARS spike glycoprotein S1 is closely associated with the pathogenesis of COVID-19. Its binding to ACE2 can lead to dysregulation of blood pressure and inflammatory responses contributing to severe respiratory syndrome. The Spike S1 protein also plays a role in the potential development of myocarditis a complication of COVID-19 where it may induce cardiac inflammation. Connections with other viral proteins including the nucleocapsid protein further implicate the Spike S1 in enhancing viral assembly and pathogenicity during infection.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SARS spike glycoprotein S1
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Product promise

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