AB286179

Anti-SARS spike glycoprotein+COVID-19 Spike Protein antibody [CR3022]

Recombinant
What is this?

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(1 Publication)

Human Recombinant Monoclonal SPIKE antibody. Suitable for ELISA and reacts with SARS-CoV-2, SARS-CoV samples. Cited in 1 publication.

View Alternative Names

2, S, Spike glycoprotein, S glycoprotein, E2, Peplomer protein

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

CR3022

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

SARS-CoV-2, SARS-CoV

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called A2103 Anti-CoV-2 & SARS-CoV S1 Antibody (Clone CR3022). A2103-200 is the same size as the 200 μg size of ab286179.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS spike glycoprotein also known as the COVID-19 spike protein is an important target in the study of coronaviruses. This protein with a mass of approximately 180–200 kDa plays an essential role in the virus's ability to infect host cells. Expressed on the surface of the virus the spike protein facilitates the viral entry by binding to the angiotensin-converting enzyme 2 (ACE2) receptors present on human cells. Through this interaction the spike protein initiates the fusion of the viral membrane with the host cell membrane enabling the virus to infiltrate the host.

Biological function summary

The spike protein performs several significant functions beyond its role in viral entry. It forms part of a larger viral structure together with other proteins that maintain the virus's shape and functionality. The spike protein is integral in host immune system activation as it contains multiple antigenic sites that elicit an immune response. These antigenic sites become targets for antibodies that neutralize the virus. Researchers often focus on this aspect to develop vaccines and therapeutic interventions as it determines the body's capacity to fight off the infection.

Pathways

The spike protein impacts both viral lifecycle and immune response pathways. During the viral lifecycle it is critical for initiating receptor-mediated endocytosis a pathway by which the virus gains cellular entry. Additionally its interaction with ACE2 links it to the renin-angiotensin system (RAS) pathway impacting cardiovascular regulation. Proteins such as TMPRSS2 which assists in priming the spike protein for membrane fusion also play a significant role within these pathways. Understanding these mechanisms contributes to the development of strategies to inhibit virus action and spread.

The spike protein is closely associated with COVID-19 the disease caused by the SARS-CoV-2 virus. Its function directly relates to the virus's ability to cause acute respiratory distress syndrome (ARDS) a severe condition common in critical COVID-19 patients. Researchers also study its potential links to cardiovascular issues as seen with co-expression and interaction with ACE2 in heart tissue. Furthermore the immune response triggered by the spike protein leads to the production of anti-spike protein antibodies which are vital components of serological tests for COVID-19 diagnostics and monitoring.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Spike glycoprotein. May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.. Spike protein S1. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.. Spike protein S2. Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states : pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.. Spike protein S2'. Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.

See full target information S

Additional targets

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS applied materials & interfaces 16:30196-30208 PubMed38814245

2024

Study of VCT-MXene Based Immunosensor for Sensitive Label-Free Impedimetric Detection of SARS-CoV-2 Spike Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola Tasić,Ivan Konjević,Alnilan Lobato,Dino Metarapi,Matjaž Finšgar,Filipa M Oliveira,Zděnek Sofer,Rui Gusmão,Xueji Zhang,Samo B Hočevar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SARS spike glycoprotein+COVID-19 Spike Protein antibody [CR3022]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise