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Rabbit Recombinant Monoclonal NS6 antibody. Suitable for WB and reacts with SARS-CoV-2 samples.


Images

Western blot - Anti-SARS2-CoV-2 ORF6 antibody [EPR24849-21] (AB286911), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
SARS-CoV-2
Tested

Tested
Tested

Species
SARS-CoV-2
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Disrupts bidirectional nucleocytoplasmic transport by interacting with the host RAE1-NUP98 complex (PubMed:33360543, PubMed:33849972). Disrupts cell nuclear import complex formation by tethering karyopherin alpha 2 and karyopherin beta 1 to the membrane (PubMed:32979938). Retention of import factors at the ER/Golgi membrane leads to a loss of transport into the nucleus (By similarity). Prevents STAT1 nuclear translocation in response to interferon signaling, thus blocking the expression of interferon stimulated genes (ISGs) that display multiple antiviral activities (PubMed:33097660). Suppresses IFN-beta production possibly by blocking IRF3 nuclear translocation (PubMed:32979938). Might induce accumulation of host HNRNPA1 (PubMed:33360543). May play a role in viral double membrane vesicles networks to enhance viral replication.

Alternative names

ORF6 protein, ORF6, Accessory protein 6, Non-structural protein 6, Protein X3, ns6

Rabbit Recombinant Monoclonal NS6 antibody. Suitable for WB and reacts with SARS-CoV-2 samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24849-21
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-SARS2-CoV-2 ORF6 antibody [EPR24849-21] (ab286911), expandable thumbnail

    Western blot - Anti-SARS2-CoV-2 ORF6 antibody [EPR24849-21] (ab286911)

    Rabbit monoclonal to SARS-CoV2 orf6 (ab286911) staining at 1/1000 dilution, shown in Green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) at 1/20000 dilution, shown in red.

    In Western blot, ab286911 was shown to bind specifically to SARS-CoV2 ORF6 protein. A band was observed at 13 kDa in HEK-293T transfected with SARS-CoV-2 orf6 cell lysates with no signal observed at this size in HEK-293T transfected with SARS-CoV-1 orf6 cell lysates.

    Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

    Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-SARS2-CoV-2 ORF6 antibody [EPR24849-21] (ab286911) at 1/1000 dilution

    Lane 1: HEK-293T transfected with SARS-CoV-2 orf6 protein cell lysate at 20 µg

    Lane 2: HEK-293T transfected with SARS-CoV-1 orf6 protein cell lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Lanes 1 - 2: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 13 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com