Mouse Polyclonal SART1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.
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Publications
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- Open biology 6:2016Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility.Applications:ICC/IFReactive species:HumanS E Taylor,J Bagnall,D Mason,R Levy,D G Fernig,V SeePubMed 27655733
- Cell death & disease 5:e10722014Multiple components of the spliceosome regulate Mcl1 activity in neuroblastoma.
- Molecular cancer therapeutics 11:119-312011A systems biology approach identifies SART1 as a novel determinant of both 5-fluorouracil and SN38 drug resistance in colorectal cancer.Applications:WBReactive species:HumanWendy L Allen,Leanne Stevenson,Vicky M Coyle,Puthen V Jithesh,Irina Proutski,Gail Carson,Michael A Gordon,Heinz-Josef D Lenz,Sandra Van Schaeybroeck,Daniel B Longley,Patrick G JohnstonPubMed 22027693
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Database link O43290
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Associated Products
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Target data
Function
Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.
Alternative names
U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSnu66, SART-1, hSART-1, SART1
Recommended products
Mouse Polyclonal SART1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Database link O43290
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SART1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 1 or RNA Binding Motif Protein 25 (RBM25) plays an essential role in RNA splicing. This protein has an approximate mass of 110 kDa and is expressed ubiquitously in various tissues with higher levels observed in testis and cancer cells. As part of the spliceosome SART1 helps facilitate the assembly of spliceosomal snRNPs specifically U4/U6-U5 tri-snRNP an important component for proper splicing events.
- Biological function summary
SART1 involves itself deeply in the regulation of mRNA maturation and stability. It participates in the spliceosome complex where it contributes to the splicing of precursor-mRNA into mature mRNA which is necessary for accurate gene expression. The presence of SART1 ensures the correct removal of introns from pre-mRNA an essential step for producing functional proteins in cells.
- Pathways
The involvement in mRNA splicing positions SART1 within the gene expression pathway. It collaborates with other proteins like SF3A1 and SF3B1 integral to the spliceosome's functioning and it also indirectly influences pathways such as the cell cycle regulation by ensuring accurate gene expression. Disruptions in pathways involving SART1 can lead to erroneous splicing and subsequent protein malfunction.
- Associated diseases and disorders
Disruptions in SART1 expression or function can relate to cancer and autoimmune diseases. Aberrant splicing due to SART1 anomalies contributes to tumorigenesis seen in several forms of cancer including colorectal cancer. Additionally SART1's dysregulation can connect to proteins like PKM where mis-splicing can influence cancer metabolism. It also plays a role in autoimmune conditions as faulty splicing can trigger immune system malfunctions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-SART1 antibody (ab88583)
All lanes: Western blot - Anti-SART1 antibody (ab88583) at 1 µg/mL
All lanes: Jurkat cell lysate at 50 µg
SecondaryAll lanes: Goat anti-Mouse IgG (H+L) HRP at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 110 kDa
Western blot - Anti-SART1 antibody (ab88583)
All lanes: Western blot - Anti-SART1 antibody (ab88583) at 1 µg/mL
Lane 1: SART1 transfected 293T cell lysate at 25 µg
Lane 2: Non-transfected lysate at 25 µg
SecondaryAll lanes: Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution
Predicted band size: 90 kDa
Observed band size: 130 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (ab88583)
ab88583 at 10μg/ml staining SART1 in HeLa cells by Immunofluorescence.
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