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AB88583

Anti-SART1 antibody

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(3 Publications)

Mouse Polyclonal SART1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSnu66, SART-1, hSART-1, SART1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (AB88583)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody (AB88583)

ab88583 at 10μg/ml staining SART1 in HeLa cells by Immunofluorescence.

Western blot - Anti-SART1 antibody (AB88583)
  • WB

Unknown

Western blot - Anti-SART1 antibody (AB88583)

All lanes:

Western blot - Anti-SART1 antibody (ab88583) at 1 µg/mL

Lane 1:

SART1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

Predicted band size: 90 kDa

Observed band size: 130 kDa

false

Western blot - Anti-SART1 antibody (AB88583)
  • WB

Unknown

Western blot - Anti-SART1 antibody (AB88583)

All lanes:

Western blot - Anti-SART1 antibody (ab88583) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) HRP at 1/50000 dilution

Predicted band size: 90 kDa

Observed band size: 110 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SART1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 1 or RNA Binding Motif Protein 25 (RBM25) plays an essential role in RNA splicing. This protein has an approximate mass of 110 kDa and is expressed ubiquitously in various tissues with higher levels observed in testis and cancer cells. As part of the spliceosome SART1 helps facilitate the assembly of spliceosomal snRNPs specifically U4/U6-U5 tri-snRNP an important component for proper splicing events.
Biological function summary

SART1 involves itself deeply in the regulation of mRNA maturation and stability. It participates in the spliceosome complex where it contributes to the splicing of precursor-mRNA into mature mRNA which is necessary for accurate gene expression. The presence of SART1 ensures the correct removal of introns from pre-mRNA an essential step for producing functional proteins in cells.

Pathways

The involvement in mRNA splicing positions SART1 within the gene expression pathway. It collaborates with other proteins like SF3A1 and SF3B1 integral to the spliceosome's functioning and it also indirectly influences pathways such as the cell cycle regulation by ensuring accurate gene expression. Disruptions in pathways involving SART1 can lead to erroneous splicing and subsequent protein malfunction.

Disruptions in SART1 expression or function can relate to cancer and autoimmune diseases. Aberrant splicing due to SART1 anomalies contributes to tumorigenesis seen in several forms of cancer including colorectal cancer. Additionally SART1's dysregulation can connect to proteins like PKM where mis-splicing can influence cancer metabolism. It also plays a role in autoimmune conditions as faulty splicing can trigger immune system malfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.
See full target information U4/U6.U5 tri-snRNP-associated protein 1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Open biology 6: PubMed27655733

2016

Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility.

Applications

ICC/IF

Species

Human

S E Taylor,J Bagnall,D Mason,R Levy,D G Fernig,V See

Cell death & disease 5:e1072 PubMed24556687

2014

Multiple components of the spliceosome regulate Mcl1 activity in neuroblastoma.

Applications

WB

Species

Unspecified reactive species

T W Laetsch,X Liu,A Vu,M Sliozberg,M Vido,O U Elci,K C Goldsmith,M D Hogarty

Molecular cancer therapeutics 11:119-31 PubMed22027693

2011

A systems biology approach identifies SART1 as a novel determinant of both 5-fluorouracil and SN38 drug resistance in colorectal cancer.

Applications

WB

Species

Human

Wendy L Allen,Leanne Stevenson,Vicky M Coyle,Puthen V Jithesh,Irina Proutski,Gail Carson,Michael A Gordon,Heinz-Josef D Lenz,Sandra Van Schaeybroeck,Daniel B Longley,Patrick G Johnston
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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