AB95957

Anti-SART1 antibody

Name: Anti-SART1 antibody (ab95957) | Abcam
Brand: Abcam Limited
SKU: AB95957
Price: 510 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SART1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSnu66, SART-1, hSART-1, SART1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SART1 antibody (AB95957)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SART1 antibody (AB95957)

ab95957, at a 1/500 dilution, staining SART1 in paraffin embedded Cal27 xenograft by Immunohistochemical analysis.

Supplier Data

Western blot - Anti-SART1 antibody (AB95957)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-SART1 antibody (ab95957) at 1/5000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

K562 whole cell extracts at 30 µg

Lane 4:

NCI-H929 whole cell extracts at 30 µg

Predicted band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SART1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 1 or RNA Binding Motif Protein 25 (RBM25) plays an essential role in RNA splicing. This protein has an approximate mass of 110 kDa and is expressed ubiquitously in various tissues with higher levels observed in testis and cancer cells. As part of the spliceosome SART1 helps facilitate the assembly of spliceosomal snRNPs specifically U4/U6-U5 tri-snRNP an important component for proper splicing events.

Biological function summary

SART1 involves itself deeply in the regulation of mRNA maturation and stability. It participates in the spliceosome complex where it contributes to the splicing of precursor-mRNA into mature mRNA which is necessary for accurate gene expression. The presence of SART1 ensures the correct removal of introns from pre-mRNA an essential step for producing functional proteins in cells.

Pathways

The involvement in mRNA splicing positions SART1 within the gene expression pathway. It collaborates with other proteins like SF3A1 and SF3B1 integral to the spliceosome's functioning and it also indirectly influences pathways such as the cell cycle regulation by ensuring accurate gene expression. Disruptions in pathways involving SART1 can lead to erroneous splicing and subsequent protein malfunction.

Disruptions in SART1 expression or function can relate to cancer and autoimmune diseases. Aberrant splicing due to SART1 anomalies contributes to tumorigenesis seen in several forms of cancer including colorectal cancer. Additionally SART1's dysregulation can connect to proteins like PKM where mis-splicing can influence cancer metabolism. It also plays a role in autoimmune conditions as faulty splicing can trigger immune system malfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.

See full target information U4/U6.U5 tri-snRNP-associated protein 1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 13:9037-9048 PubMed34916848

2021

LncRNA Promotes Tumorigenesis of Glioma via Targeting miR-128-3p/ Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Li,Ming Li,Pengcheng Xia,Lili Wang,Zhiming Lu

PloS one 13:e0193477 PubMed29481555

2018

The two glycolytic markers GLUT1 and MCT1 correlate with tumor grade and survival in clear-cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Damien Ambrosetti,Maeva Dufies,Bérengère Dadone,Matthieu Durand,Delphine Borchiellini,Jean Amiel,Jacques Pouyssegur,Nathalie Rioux-Leclercq,Gilles Pages,Fanny Burel-Vandenbos,Nathalie M Mazure

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SART1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SART1 antibody (AB95957)

Western blot - Anti-SART1 antibody (AB95957)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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