AB181867

Anti-SART1 antibody [EPR14563(B)]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SART1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSnu66, SART-1, hSART-1, SART1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody [EPR14563(B)] (AB181867)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody [EPR14563(B)] (AB181867)

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling SART1 with ab181867 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] (AB181867)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] (AB181867)

Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SART1 with ab181867 at 1/150 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Western blot - Anti-SART1 antibody [EPR14563(B)] (AB181867)

All lanes:

Western blot - Anti-SART1 antibody [EPR14563(B)] (ab181867) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

MOLT4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 90 kDa

false

Carrier free

Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14563(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SART1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 1 or RNA Binding Motif Protein 25 (RBM25) plays an essential role in RNA splicing. This protein has an approximate mass of 110 kDa and is expressed ubiquitously in various tissues with higher levels observed in testis and cancer cells. As part of the spliceosome SART1 helps facilitate the assembly of spliceosomal snRNPs specifically U4/U6-U5 tri-snRNP an important component for proper splicing events.

Biological function summary

SART1 involves itself deeply in the regulation of mRNA maturation and stability. It participates in the spliceosome complex where it contributes to the splicing of precursor-mRNA into mature mRNA which is necessary for accurate gene expression. The presence of SART1 ensures the correct removal of introns from pre-mRNA an essential step for producing functional proteins in cells.

Pathways

The involvement in mRNA splicing positions SART1 within the gene expression pathway. It collaborates with other proteins like SF3A1 and SF3B1 integral to the spliceosome's functioning and it also indirectly influences pathways such as the cell cycle regulation by ensuring accurate gene expression. Disruptions in pathways involving SART1 can lead to erroneous splicing and subsequent protein malfunction.

Disruptions in SART1 expression or function can relate to cancer and autoimmune diseases. Aberrant splicing due to SART1 anomalies contributes to tumorigenesis seen in several forms of cancer including colorectal cancer. Additionally SART1's dysregulation can connect to proteins like PKM where mis-splicing can influence cancer metabolism. It also plays a role in autoimmune conditions as faulty splicing can trigger immune system malfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.

See full target information U4/U6.U5 tri-snRNP-associated protein 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:575 PubMed40830790

2025

Engineered MEVs for photoreceptor-targeted delivery of USP25 to alleviate diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxiang Sun,Shenyuan Chen,Xiaoyuan Qi,Yuntong Sun,Zhengmei Jiang,Jie Chang,Yongjun Ma,Jin Huang,Benshuai You,Fengtian Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SART1 antibody [EPR14563(B)]

Immunocytochemistry/ Immunofluorescence - Anti-SART1 antibody [EPR14563(B)] (AB181867)

Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] (AB181867)

Western blot - Anti-SART1 antibody [EPR14563(B)] (AB181867)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise