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Rabbit Recombinant Monoclonal SART1 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human samples.

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Images

Western blot - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (AB250536), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (AB250536), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IFWB
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.

    Alternative names

    U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSnu66, SART-1, hSART-1, SART1

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    Rabbit Recombinant Monoclonal SART1 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human samples.

    Alternative names

    U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSnu66, SART-1, hSART-1, SART1

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR14563(B)

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Storage information

    Do Not Freeze

    Notes

    ab250536 is the carrier-free version of Anti-SART1 antibody [EPR14563(B)] ab181867.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    SART1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 1 or RNA Binding Motif Protein 25 (RBM25) plays an essential role in RNA splicing. This protein has an approximate mass of 110 kDa and is expressed ubiquitously in various tissues with higher levels observed in testis and cancer cells. As part of the spliceosome SART1 helps facilitate the assembly of spliceosomal snRNPs specifically U4/U6-U5 tri-snRNP an important component for proper splicing events.

    Biological function summary

    SART1 involves itself deeply in the regulation of mRNA maturation and stability. It participates in the spliceosome complex where it contributes to the splicing of precursor-mRNA into mature mRNA which is necessary for accurate gene expression. The presence of SART1 ensures the correct removal of introns from pre-mRNA an essential step for producing functional proteins in cells.

    Pathways

    The involvement in mRNA splicing positions SART1 within the gene expression pathway. It collaborates with other proteins like SF3A1 and SF3B1 integral to the spliceosome's functioning and it also indirectly influences pathways such as the cell cycle regulation by ensuring accurate gene expression. Disruptions in pathways involving SART1 can lead to erroneous splicing and subsequent protein malfunction.

    Associated diseases and disorders

    Disruptions in SART1 expression or function can relate to cancer and autoimmune diseases. Aberrant splicing due to SART1 anomalies contributes to tumorigenesis seen in several forms of cancer including colorectal cancer. Additionally SART1's dysregulation can connect to proteins like PKM where mis-splicing can influence cancer metabolism. It also plays a role in autoimmune conditions as faulty splicing can trigger immune system malfunctions.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    2 product images

    • Western blot - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (ab250536), expandable thumbnail

      Western blot - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (ab250536)

      This data was developed using Anti-SART1 antibody [EPR14563(B)] ab181867, the same antibody clone in a different buffer formulation.

      All lanes: Western blot - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (ab250536) at 1/50000 dilution

      Lane 1: HeLa cell lysate at 20 µg

      Lane 2: Jurkat cell lysate at 20 µg

      Lane 3: MOLT4 cell lysate at 20 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 90 kDa

    • Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (ab250536), expandable thumbnail

      Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (ab250536)

      This data was developed using Anti-SART1 antibody [EPR14563(B)] ab181867, the same antibody clone in a different buffer formulation.

      Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SART1 with Anti-SART1 antibody [EPR14563(B)] ab181867 at 1/150 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com