AB250536

Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SART1 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSnu66, SART-1, hSART-1, SART1

2 Images

Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (AB250536)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (AB250536)

This data was developed using ab181867, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SART1 with ab181867 at 1/150 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Western blot - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (AB250536)

This data was developed using ab181867, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (ab250536) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

MOLT4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 90 kDa

false

Unconjugated

Anti-SART1 antibody [EPR14563(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14563(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250536 is the carrier-free version of ab181867.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SART1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 1 or RNA Binding Motif Protein 25 (RBM25) plays an essential role in RNA splicing. This protein has an approximate mass of 110 kDa and is expressed ubiquitously in various tissues with higher levels observed in testis and cancer cells. As part of the spliceosome SART1 helps facilitate the assembly of spliceosomal snRNPs specifically U4/U6-U5 tri-snRNP an important component for proper splicing events.

Biological function summary

SART1 involves itself deeply in the regulation of mRNA maturation and stability. It participates in the spliceosome complex where it contributes to the splicing of precursor-mRNA into mature mRNA which is necessary for accurate gene expression. The presence of SART1 ensures the correct removal of introns from pre-mRNA an essential step for producing functional proteins in cells.

Pathways

The involvement in mRNA splicing positions SART1 within the gene expression pathway. It collaborates with other proteins like SF3A1 and SF3B1 integral to the spliceosome's functioning and it also indirectly influences pathways such as the cell cycle regulation by ensuring accurate gene expression. Disruptions in pathways involving SART1 can lead to erroneous splicing and subsequent protein malfunction.

Disruptions in SART1 expression or function can relate to cancer and autoimmune diseases. Aberrant splicing due to SART1 anomalies contributes to tumorigenesis seen in several forms of cancer including colorectal cancer. Additionally SART1's dysregulation can connect to proteins like PKM where mis-splicing can influence cancer metabolism. It also plays a role in autoimmune conditions as faulty splicing can trigger immune system malfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.

See full target information U4/U6.U5 tri-snRNP-associated protein 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (AB250536)

Western blot - Anti-SART1 antibody [EPR14563(B)] - BSA and Azide free (AB250536)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise