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AB111901

Anti-SASH1 antibody

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(2 Publications)

Rabbit Polyclonal SASH1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SASH1 aa 750-800.

View Alternative Names

KIAA0790, PEPE1, SASH1, SAM and SH3 domain-containing protein 1, Proline-glutamate repeat-containing protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH1 antibody (AB111901)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH1 antibody (AB111901)

ab111901 at 1/250 dilution staining SASH1 in Formalin-fixed, Paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry. Detection : DAB staining

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH1 antibody (AB111901)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH1 antibody (AB111901)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SASH1 with ab111901 at 1/100 dilution, and detection antibody Red fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated at 1/100 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SASH1 aa 750-800. The exact immunogen used to generate this antibody is proprietary information.

O94885

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111901 is affinity purified using an epitope specific to SASH1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SASH1 protein also known as SAM and SH3 domain-containing 1 weighs approximately 137 kDa. Researchers find it expressed in various tissues with higher levels noted in the brain and some epithelial tissues. Mechanically SASH1 functions as an adaptor protein interacting with other proteins through its SAM and SH3 domains. These interactions are critical for SASH1's role in organizing protein complexes and regulating various cellular processes.
Biological function summary

SASH1 influences cell migration adhesion and cytoskeletal organization playing an important role in maintaining normal cellular architecture and function. It does not form part of a known larger protein complex but it acts by connecting signaling molecules essential for cytoskeleton dynamics. Its influence on cellular architecture makes SASH1 an important link between signaling pathways and structural cellular changes.

Pathways

This protein has been found to modulate processes in the Wnt and PI3K/Akt pathways. These pathways are critical for regulating cell growth and survival and SASH1 serves to bridge signaling molecules required for effective pathway function. Its relation to proteins such as β-catenin and Akt places it centrally in controlling cellular responses to external stimuli impacting cell fate decisions.

Research associates SASH1 with certain cancers such as breast cancer and with cardiovascular diseases. Aberrant expression or mutations of SASH1 can influence cancer progression or cardiovascular conditions by altering cell migration and adhesion. Its interaction with proteins like E-cadherin and integrins highlights its importance in maintaining proper cellular adhesion and disruptions in these interactions often correlate with disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a positive regulator of NF-kappa-B signaling downstream of TLR4 activation. It acts as a scaffold molecule to assemble a molecular complex that includes TRAF6, MAP3K7, CHUK and IKBKB, thereby facilitating NF-kappa-B signaling activation (PubMed : 23776175). Regulates TRAF6 and MAP3K7 ubiquitination (PubMed : 23776175). Involved in the regulation of cell mobility (PubMed : 23333244, PubMed : 23776175, PubMed : 25315659). Regulates lipolysaccharide (LPS)-induced endothelial cell migration (PubMed : 23776175). Is involved in the regulation of skin pigmentation through the control of melanocyte migration in the epidermis (PubMed : 23333244).
See full target information SASH1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Chinese medical journal 136:1177-1187 PubMed37083129

2023

Single-cell RNA sequencing reveals the transcriptomic landscape of kidneys in patients with ischemic acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Tang,Peng Jin,Chanjuan Shen,Wei Lin,Leilin Yu,Xueling Hu,Ting Meng,Linlin Zhang,Ling Peng,Xiangcheng Xiao,Peter Eggenhuizen,Joshua D Ooi,Xueqin Wu,Xiang Ding,Yong Zhong

The Journal of experimental medicine 162:117-27 PubMed3159821

1985

Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

W A Jefferies,J R Green,A F Williams
View all publications
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Product promise

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