Rabbit Polyclonal SASH3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SASH3 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SASH3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75995
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Target data
Function
May function as a signaling adapter protein in lymphocytes.
Alternative names
CXorf9, SLY, SASH3, SAM and SH3 domain-containing protein 3, SH3 protein expressed in lymphocytes homolog
Recommended products
Rabbit Polyclonal SASH3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SASH3 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SASH3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75995
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SASH3 also known as SAM and SH3 Domain Containing 3 or FYVE and coiled-coil domain autoantigen 1 (FCA1) is an adaptor protein with mass approximately 138 kDa. This protein plays a mechanical role in intracellular signaling through its SH3 and SAM domains which mediate protein-protein interactions. SASH3 is expressed mainly in lymphoid tissues including spleen and thymus making it significant in the immune system.
- Biological function summary
SASH3 functions in cellular signaling processes related to immune response. SASH3 is not part of a large protein complex but associates with other proteins in immune cells to regulate signaling pathways. It plays a role in modulating T-cell activation a critical function for orchestrating immune responses.
- Pathways
SASH3 interfaces notably with the T-cell receptor signaling pathway influencing immune cell activation. It interacts with proteins such as Vav1 and Lck which are known to mediate signal transduction in response to activation of the immune system. SASH3's involvement in these pathways impacts cellular proliferation and differentiation core elements of immune response control.
- Associated diseases and disorders
SASH3 relates to conditions such as systemic lupus erythematosus and other autoimmune diseases where improper immune regulation occurs. Altered SASH3 expression or mutations may contribute to these diseases by disrupting normal signal transduction involving related proteins like CD3 and ZAP-70. Understanding SASH3's function could offer insights for developing therapeutic targets in treating such immune system disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-SASH3 antibody (ab251656)
SASH3 Western blot staining using rabbit Anti-SASH3 antibody
All lanes: Western blot - Anti-SASH3 antibody (ab251656) at 0.4 µg/mL
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2: HEK-293T overexpressing human SASH3 with a C-terminal myc-DDK tag, whole cell lysate
Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH3 antibody (ab251656)
Paraffin-embedded human duodenum tissue stained for SASH3 using ab251656 in immunohistochemical analysis, showing moderate cytoplasmic positivity in lymphoid cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH3 antibody (ab251656)
Paraffin-embedded human testis tissue stained for SASH3 using ab251656 in immunohistochemical analysis, showing no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH3 antibody (ab251656)
Paraffin-embedded human lymph node tissue stained for SASH3 using ab251656 in immunohistochemical analysis, showing moderate cytoplasmic positivity in lymphocytes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SASH3 antibody (ab251656)
Paraffin-embedded human skeletal muscle tissue stained for SASH3 using ab251656 at 1/50 dilution in immunohistochemical analysis. Low expression, as expected.
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