AB85993

Anti-SASS6 antibody

Name: Anti-SASS6 antibody (ab85993) | Abcam
Brand: Abcam Limited
SKU: AB85993
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SASS6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SASS6 aa 600 to C-terminus.

View Alternative Names

SAS6, SASS6, Spindle assembly abnormal protein 6 homolog, HsSAS-6, Spindle assembly defective protein 6

2 Images

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Western blot - Anti-SASS6 antibody (AB85993)

All lanes:

Western blot - Anti-SASS6 antibody (ab85993) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 74 kDa

Observed band size: 74 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-SASS6 antibody (AB85993)

Detection of Human SASS6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85993 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SASS6 antibody (ab85993)

Predicted band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SASS6 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6UVJ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SASS6 also known as spindle assembly 6 homolog is a protein that plays an important role in centriole biogenesis. It has a molecular mass of approximately 82 kDa. SASS6 expresses not only in dividing cells but also in tissues where active cell division takes place including the brain testis and other proliferative tissues. Its distribution indicates its involvement in cellular processes that require precise organelle replication and segregation.

Biological function summary

SASS6 integrates into a complex essential for centriole duplication which is fundamental for accurate mitotic spindle formation. It forms a structural core in the assembling centrioles ensuring their cylindrical shape. The protein helps in organizing microtubule triplets around a central hub which is vital for proper cell division and maintenance of genomic stability.

Pathways

The protein contributes significantly to the cell cycle regulation and centrosome cohesion. It participates in the centriole duplication pathway which interacts with other proteins like STIL and CEP135. Through this interaction SASS6 ensures efficient progression and regulation within the centrosome cycle which is critical for cell cycle checkpoints and prevention of aneuploidy.

Mutations or dysregulation of SASS6 are linked to microcephaly and other developmental anomalies. Such conditions often result from improper centriole formation impacting neurogenesis and brain size. The involvement of SASS6 also cross-references with proteins such as CEP63 in these disorders highlighting a shared pathway impacting centrosome-related abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Central scaffolding component of the centrioles ensuring their 9-fold symmetry (By similarity). Required for centrosome biogenesis and duplication : required both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multiciliated cells (PubMed : 15665853, PubMed : 16244668, PubMed : 17681131). Not required for centriole formation in embryonic stem cells but necessary to maintain centriole architecture (By similarity). Required for the recruitment of STIL to the procentriole and for STIL-mediated centriole amplification (PubMed : 22020124). Overexpression results in excess foci-bearing centriolar markers (PubMed : 15665853).

See full target information SASS6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:760999 PubMed34804049

2021

Inhibitor of Differentiation-2 Protein Ameliorates DSS-Induced Ulcerative Colitis by Inhibiting NF-κB Activation in Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Dong Yan,Yichun Wang,Jiaojiao Zhang,Min Li,Wancheng Xiong,Xueqian Jing,Puze Li,Weidong Zhao,Xiwen Xiong,Minna Wu,Genshen Zhong

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SASS6 antibody

Western blot - Anti-SASS6 antibody (AB85993)

Immunoprecipitation - Anti-SASS6 antibody (AB85993)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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