AB69995

Anti-SATB2 antibody

Name: Anti-SATB2 antibody (ab69995) | Abcam
Brand: Abcam Limited
SKU: AB69995
Price: 525 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(6 Publications)

Rabbit Polyclonal SATB2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SATB2.

View Alternative Names

KIAA1034, SATB2, DNA-binding protein SATB2, Special AT-rich sequence-binding protein 2

1 Images

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Western blot - Anti-SATB2 antibody (AB69995)

Lane 1:

Western blot - Anti-SATB2 antibody (ab69995) at 2 µg/mL

Lane 2:

Western blot - Anti-SATB2 antibody (ab69995) at 4 µg/mL

All lanes:

Mouse brain tissue lysate at 15 µg

Predicted band size: 83 kDa

Observed band size: 90 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human SATB2. The exact immunogen used to generate this antibody is proprietary information.

Q9UPW6

Specificity

ab69995 does not cross-react with SATB1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SATB2 protein also known as Special AT-rich Sequence-Binding Protein 2 is a DNA-binding protein with a mass of about 82 kDa. It plays a role in chromatin organization and transcriptional regulation by binding to AT-rich sequences within the nuclear matrix. SATB2 shows high expression levels in the cerebral cortex and the bone where it influences gene expression. It functions by facilitating the assembly of transcriptional machineries enhancing or repressing the transcription of its target genes.

Biological function summary

SATB2 acts as a regulator of multiple developmental processes. It serves as a master control gene in osteoblast differentiation and skeletal development by forming complexes with core binding factors. In the brain SATB2 contributes to the development and maintenance of neuronal networks that are essential for cognitive functions. Its binding to matrix attachment regions aids in the spatial organization of nuclear chromatin impacting gene expression pertinent to development and differentiation.

Pathways

SATB2 operates within the Wnt signaling pathway and the regulation of osteoblast differentiation. It interacts with proteins such as RUNX2 and β-catenin which play roles in bone formation and homeostasis. Within these pathways SATB2 modulates gene networks that influence the expression of structural and signaling proteins important for tissue differentiation and repair particularly in bone and neuronal contexts.

SATB2 has associations with cleft palate and intellectual disabilities conditions often related to alterations in craniofacial and neurological development. It interacts with proteins like SOX9 and TCF7L1 which have roles in developmental processes that when disrupted can lead to such conditions. Understanding SATB2's involvement in these disorders provides insights into potential therapeutic targets for interventions aiming to mitigate developmental abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

See full target information SATB2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35506651

2022

Generation of vascularized brain organoids to study neurovascular interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yao Sun,Xiang-Chun Ju,Yang Li,Peng-Ming Zeng,Jian Wu,Ying-Ying Zhou,Li-Bing Shen,Jian Dong,Yue-Jun Chen,Zhen-Ge Luo

Cell reports 35:109290 PubMed34192541

2021

The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Yi Ou,Qi Xiao,Xiang-Chun Ju,Peng-Ming Zeng,Jing Huang,Ai-Li Sheng,Zhen-Ge Luo

Nature communications 10:2966 PubMed31273213

2019

Mutations in SMARCB1 and in other Coffin-Siris syndrome genes lead to various brain midline defects.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Filatova,Linda K Rey,Marion B Lechler,Jörg Schaper,Maja Hempel,Renata Posmyk,Krzysztof Szczaluba,Gijs W E Santen,Dagmar Wieczorek,Ulrike A Nuber

Experimental and therapeutic medicine 14:3507-3512 PubMed29042940

2017

Expression and localization of special AT-rich sequence binding protein 2 in murine molar development and the pulp-dentin complex of human healthy teeth and teeth with pulpitis.

Applications

Unspecified application

Species

Unspecified reactive species

Lina He,Huimei Liu,Lei Shi,Shuang Pan,Xu Yang,Lin Zhang,Yumei Niu

Cellular and molecular neurobiology 35:205-16 PubMed25239161

2014

Satb2-independent acquisition of the cholinergic sudomotor phenotype in rodents.

Applications

Unspecified application

Species

Rat

Burkhard Schütz,Martin K-H Schäfer,Markus Gördes,Lee E Eiden,Eberhard Weihe

Tissue engineering. Part A 17:1767-76 PubMed21385070

2011

Roles of SATB2 in osteogenic differentiation and bone regeneration.

Applications

IHC-P

Species

Mouse

Jin Zhang,Qisheng Tu,Rudolf Grosschedl,Min Seok Kim,Terrence Griffin,Hicham Drissi,Pishan Yang,Jake Chen

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