Name: Anti-SATB2 antibody [EPNCIR130A]
SKU: ab92446
Rating: 5 (9 reviews)

Anti-SATB2 antibody [EPNCIR130A] is a rabbit monoclonal antibody that is used in SATB2 western blot (WB), IHC, immunocytochemistry/immunofluorescence (ICC/IF), and flow cytometry. Suitable for human, mouse, and rat samples.

- Cited in over 70 publications
- Recombinant format for unrivaled batch-batch consistency
- Specificity confirmed with SATB2 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Images

Western blot - Anti-SATB2 antibody [EPNCIR130A] (AB92446), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Not recommended	Tested	Tested	Tested
Mouse	Expected	Not recommended	Tested	Expected	Expected
Rat	Expected	Not recommended	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/150 - 1/300	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes -
Species Rat	Dilution info 1/1000 - 1/5000	Notes -
Species Human	Dilution info 1/1000 - 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information SATB2

Function

Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

Alternative names

KIAA1034, SATB2, DNA-binding protein SATB2, Special AT-rich sequence-binding protein 2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPNCIR130A
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of John Niederhuber. View antibodies from NCI Center for Cancer Research Collaboration.

Product Specifications
Anti-SATB2 antibody [EPNCIR130A] (ab92446) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-SATB2 antibody [EPNCIR130A] (ab92446) specifically detects SATB2 (UniProt ID: Q9UPW6; Molecular weight: 83kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-SATB2 antibody [EPNCIR130A] (ab92446) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-SATB2 antibody [EPNCIR130A] (ab92446) has been confirmed by testing in knockout samples.
Anti-SATB2 antibody [EPNCIR130A] (ab92446) has been cited over 73 times in peer reviewed journals and is trusted by the scientific community.
Anti-SATB2 antibody [EPNCIR130A] (ab92446) has 8 independent reviews from customers.

Related Products
Antibody clone EPNCIR130A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 594, APC, PE (ab196536, ab207040, ab225051, ab225052).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Lanes 1 - 2: Merged signal (red and green). Green - ab92446 observed at 83 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab92446 was shown to specifically react with SATB2 in wild-type HAP1 cells as signal was lost in SATB2 knockout cells. Wild-type and SATB2 knockout samples were subjected to SDS-PAGE. ab92446 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: SATB2 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 83 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SATB2 with purified ab92446 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
All lanes: SW1353 (human chondrosarcoma cell line) cell lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
Lane 1: Saos-2 (human osteosarcoma cell line) cell lysate at 20 µg
Lane 2: Human fetal brain tissue lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
All lanes: Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
Lane 1: HT1080 (human fibrosarcoma cell line) cell lysate at 10 µg
Lane 2: SW1353 (human chondrosarcoma cell line) cell lysate at 10 µg
Lane 3: MCF7 (human breast adenocarcinoma cell line) cell lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Immunocytochemistry/Immunofluorescence analysis of Saos-2 cells (human osteosarcoma cell line) labelling SATB2 (red) with purified ab92446 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083, an Alexa Fluor® 647-conjugated goat anti-rabbit IgG H&L (1/1000), was used as the secondary antibody. Tubulin (green) was stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, an anti-alpha tubulin antibody (1/1000). DAPI (blue) was used as the nuclear counterstain.
Immunohistochemistry - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling SATB2 with ab92446 at a concentration of 2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 56mins.
ab92446 anti-SATB2 [EPNCIR130A] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Freshly made lysate is highly recommended to minimize protein degradation.
All lanes: Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/10000 dilution
Lane 1: Saos-2 (Human osteosarcoma epithelial) whole cell fresh lysate at 10 µg
Lane 2: Saos-2 (Human osteosarcoma epithelial) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 20s
Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
False colour image of Western blot: Anti-SATB2 antibody [EPNCIR130A] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92446 was shown to bind specifically to SATB2. A band was observed at 100 kDa in wild-type HAP1 cell lysates with no signal observed at this size in SATB2 knockout cell line. To generate this image, wild-type and SATB2 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
Lanes 1, 10, 2, 3, 4, 5, 6, 7, 8 and 9: Western blot - Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
Lanes 1, 10, 2, 3, 4, 5, 6, 7, 8 and 9: Western blot - Anti-SATB2 antibody [EPNCIR130A] - BSA and Azide free (Anti-SATB2 antibody [EPNCIR130A] - BSA and Azide free ab212177) at 1/1000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: SATB2 knockout HAP1 cell lysate at 20 µg
Lane 3: Mouse E18 Embyonic brain cell lysate at 20 µg
Lane 4: NIH/3T3 cell lysate at 20 µg
Lane 5: HT1080 cell lysate at 20 µg
Lane 6: Saos-2 cell lysate at 20 µg
Lanes 7 and 9: Empty
Lane 8: SATB1 Recombinant Protein cell lysate at 0.1 µg
Lane 10: SATB2 Recombinant Protein (Recombinant Human SATB2 protein ab132405) cell lysate at 0.1 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 100 kDa
Flow Cytometry (Intracellular) - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Intracellular Flow Cytometry analysis of SH-SY5Y cells (human neuroblastoma cell line from bone marrow) labeling SATB2 with purified ab92446 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry - Anti-SATB2 antibody [EPNCIR130A] (ab92446)
Immunohistochemical analysis of formalin fixed paraffin embedded human cortex labelling SATB2 with ab92446 at a concentration of 2 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 56 mins.
ab92446 anti-SATB2 [EPNCIR130A] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)