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Rabbit Recombinant Monoclonal SATB2 antibody. C-terminal. Suitable for WB, IHC-FoFr, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (AB227686), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (AB227686), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (AB227686), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (AB227686), expandable thumbnail
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SATB2 antibody [SP281] - C-terminal (AB227686), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-FoFrIHC-PICC/IF
Human
Not recommended
Tested
Expected
Tested
Not recommended
Mouse
Not recommended
Expected
Tested
Tested
Not recommended
Rat
Not recommended
Expected
Expected
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate for 10 minutes at room temperature.

Species
Rat
Dilution info
1/100
Notes

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate for 10 minutes at room temperature.

Species
Human
Dilution info
1/100
Notes

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate for 10 minutes at room temperature.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

Alternative names

KIAA1034, SATB2, DNA-binding protein SATB2, Special AT-rich sequence-binding protein 2

Recommended products

Rabbit Recombinant Monoclonal SATB2 antibody. C-terminal. Suitable for WB, IHC-FoFr, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SP281
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SATB2 protein also known as Special AT-rich Sequence-Binding Protein 2 is a DNA-binding protein with a mass of about 82 kDa. It plays a role in chromatin organization and transcriptional regulation by binding to AT-rich sequences within the nuclear matrix. SATB2 shows high expression levels in the cerebral cortex and the bone where it influences gene expression. It functions by facilitating the assembly of transcriptional machineries enhancing or repressing the transcription of its target genes.

Biological function summary

SATB2 acts as a regulator of multiple developmental processes. It serves as a master control gene in osteoblast differentiation and skeletal development by forming complexes with core binding factors. In the brain SATB2 contributes to the development and maintenance of neuronal networks that are essential for cognitive functions. Its binding to matrix attachment regions aids in the spatial organization of nuclear chromatin impacting gene expression pertinent to development and differentiation.

Pathways

SATB2 operates within the Wnt signaling pathway and the regulation of osteoblast differentiation. It interacts with proteins such as RUNX2 and β-catenin which play roles in bone formation and homeostasis. Within these pathways SATB2 modulates gene networks that influence the expression of structural and signaling proteins important for tissue differentiation and repair particularly in bone and neuronal contexts.

Associated diseases and disorders

SATB2 has associations with cleft palate and intellectual disabilities conditions often related to alterations in craniofacial and neurological development. It interacts with proteins like SOX9 and TCF7L1 which have roles in developmental processes that when disrupted can lead to such conditions. Understanding SATB2's involvement in these disorders provides insights into potential therapeutic targets for interventions aiming to mitigate developmental abnormalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling SATB2 with ab227686 at 1/100 dilution (1.58 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227686 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling SATB2 with ab227686 at 1/100 dilution (1.58 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the mouse colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227686 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling SATB2 with ab227686 at 1/100 dilution (1.58 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the human colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227686 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling SATB2 with ab227686 at 1/100 dilution (1.58 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227686 for 10 mins at room temperature.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of mouse cerebral cortex tissue sections labeling SATB2 with purified ab227686 at 1:20 (7.9 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for SATB2 using ab227686 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Western blot - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    All lanes: Western blot - Anti-SATB2 antibody [SP281] - C-terminal (ab227686) at 1/400 dilution

    All lanes: HT1080 (human fibrosarcoma cell line) cell lysate

    Predicted band size: 83 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for SATB2 using ab227686 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [SP281] - C-terminal (ab227686)

    Formalin-fixed, paraffin-embedded human colon tissue stained for SATB2 using ab227686 at 1/100 dilution in immunohistochemical analysis.

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