AB230265

Anti-SAV1 antibody

Name: Anti-SAV1 antibody (ab230265) | Abcam
Brand: Abcam Limited
SKU: AB230265
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SAV1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAV1 aa 1-250.

View Alternative Names

WW45, SAV1, Protein salvador homolog 1, 45 kDa WW domain protein, hWW45

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SAV1 antibody (AB230265)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAV1 antibody (AB230265)

A549 (human lung carcinoma cell line) cells stained for SAV1 (green) using ab230265 at 1/100 dilution in ICC/IF. Secondary used is an Alexa Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAV1 antibody (AB230265)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAV1 antibody (AB230265)

Paraffin-embedded human kidney tissue stained for SAV1 using ab230265 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAV1 antibody (AB230265)

Paraffin-embedded human endometrial cancer tissue stained for SAV1 using ab230265 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SAV1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H4B6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAV1 also known as Salvador homolog 1 is a scaffold protein that plays an important role in regulating cell proliferation and apoptosis. This protein has a molecular weight of around 45 kDa and is widely expressed in various tissues throughout the body including the lung liver and heart. SAV1 contains two WW domains which facilitate interactions with other proteins critical for its function in signaling pathways.

Biological function summary

SAV1 participates in the Hippo signaling pathway acting as a core component in the regulation of organ size by controlling cell growth and apoptosis. SAV1 forms a complex with other proteins such as MST1/2 kinases and LATS1/2 kinases promoting phosphorylation events that lead to the inhibition of the transcription co-activators YAP and TAZ. This inhibition prevents unchecked cell growth proving important for maintaining proper cellular homeostasis.

Pathways

SAV1 is essential in both the Hippo and MAPK signaling pathways. In the Hippo pathway it interacts closely with MST1/2 playing a role in pathway activation and signaling cascade propagation. In the MAPK pathway it indirectly influences cellular responses to external stress signals linking to proteins such as RAF1. The coordination within these pathways ensures regulated cell proliferation and stress response.

SAV1 is linked to cancer and cardiac hypertrophy. When the Hippo pathway malfunctions due to altered SAV1 expression it can lead to uncontrolled cell growth and tumorigenesis. In some cancer types like hepatocellular carcinoma SAV1 dysfunction correlates with aggressive tumor traits. Moreover its alteration affects cardiac hypertrophy through its interactions with proteins like YAP influencing heart muscle enlargement due to stress which can lead to heart failure.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed : 29063833). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.

See full target information SAV1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:10568-10589 PubMed37739411

2023

The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Wang,Patrik Risteski,Yang Yang,Huan Chen,Gaith Droby,Andrea Walens,Deepika Jayaprakash,Melissa Troester,Laura Herring,Jonathan Chernoff,Iva M Tolić,Jessica Bowser,Cyrus Vaziri

Cell death & disease 10:374 PubMed31076566

2019

Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Han,Lin Zhang,Xiao Yu,Shidan Wang,Chunyan Xu,Heng Yin,Shujing Wang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SAV1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SAV1 antibody (AB230265)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAV1 antibody (AB230265)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAV1 antibody (AB230265)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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