Anti-SAV1 antibody [EPR26085-80] - BSA and Azide free

• WB

Lab

Western blot - Anti-SAV1 antibody [EPR26085-80] - BSA and Azide free (AB307699)

This data was developed using ab307698, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : 786-O (PMID : 12202036). The blot was developed using a high sensitivity ECL substrate. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SAV1 antibody [EPR26085-80] (<a href='/en-us/products/primary-antibodies/sav1-antibody-epr26085-80-ab307698'>ab307698</a>) at 1/1000 dilution

Lane 1:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

786-O (Human kidney renal cell adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-SAV1 antibody [EPR26085-80] - BSA and Azide free (AB307699)

This data was developed using ab307698, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The blot was developed using a high sensitivity ECL substrate. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SAV1 antibody [EPR26085-80] (<a href='/en-us/products/primary-antibodies/sav1-antibody-epr26085-80-ab307698'>ab307698</a>) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-SAV1 antibody [EPR26085-80] - BSA and Azide free (AB307699)

This data was developed using ab307698, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 11027580). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. The blot was developed using a high sensitivity ECL substrate. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SAV1 antibody [EPR26085-80] (<a href='/en-us/products/primary-antibodies/sav1-antibody-epr26085-80-ab307698'>ab307698</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-SAV1 antibody [EPR26085-80] - BSA and Azide free (AB307699)

This data was developed using ab307698, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This target was tested in fresh and frozen NIH/3T3, and it was concluded that SAV1 is the easy target for degradation. Lane 2 was freshly made and used for Western blotting immediately to minimize protein degradation. The blot was developed using a high sensitivity ECL substrate. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SAV1 antibody [EPR26085-80] (<a href='/en-us/products/primary-antibodies/sav1-antibody-epr26085-80-ab307698'>ab307698</a>) at 1/1000 dilution

Lane 1:

Frozen NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

Fresh NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

true

Exposure time: 180s