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AB99951

Anti-SBCAD antibody

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(2 Publications)

Rabbit Polyclonal SBCAD antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial aa 150 to C-terminus.

View Alternative Names

SBCAD, 2-methyl branched chain acyl-CoA dehydrogenase, 2-methylbutyryl-coenzyme A dehydrogenase, 2-MEBCAD, 2-methylbutyryl-CoA dehydrogenase, ACADSB

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (AB99951)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (AB99951)

ab99951, at 1/100 dilution, staining SBCAD in human fetal colon.

Western blot - Anti-SBCAD antibody (AB99951)
  • WB

Unknown

Western blot - Anti-SBCAD antibody (AB99951)

All lanes:

Western blot - Anti-SBCAD antibody (ab99951) at 1/500 dilution

All lanes:

Human fetal liver lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P45954

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SBCAD also known as short/branched-chain acyl-CoA dehydrogenase is an enzyme involved in the mitochondrial fatty acid beta-oxidation pathway. It has a molecular mass of approximately 42 kDa. The enzyme functions to catalyze the initial step of the beta-oxidation process specifically targeting short and branched-chain acyl-CoA substrates. SBCAD shows widespread expression notably in tissues with high fatty acid metabolism such as the liver heart and skeletal muscle.
Biological function summary

This enzyme plays a critical role in energy production by facilitating the breakdown of fatty acids to produce acetyl-CoA which subsequently enters the citric acid cycle for ATP generation. SBCAD is not a part of a larger enzyme complex but works in conjunction with other enzymes in the beta-oxidation pathway. Its function is essential for maintaining energy homeostasis particularly during periods when carbohydrates may be less available and the body relies on stored fats.

Pathways

The main involvement of SBCAD is in the fatty acid degradation and energy metabolism pathways. It is closely related to other acyl-CoA dehydrogenases such as medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VLCAD) which also participate in the stepwise oxidation of different acyl-CoA derivatives. Together these enzymes ensure efficient fatty acid breakdown and energy release through the coordinated action of the beta-oxidation cycle leading into the citric acid cycle.

Mutations in the SBCAD gene can lead to short/branched-chain acyl-CoA dehydrogenase deficiency a metabolic disorder characterized by an inability to properly break down certain types of fatty acids. This condition can result in various symptoms including muscle weakness hypoglycemia and metabolic crises often aggravated by fasting or illness. SBCAD's function interlinks with other metabolic enzymes where its deficiency might show overlapping symptoms with disorders involving MCAD and other related enzymes in the beta-oxidation sequence. Understanding SBCAD’s role is important in diagnosing and managing metabolic conditions linked to fatty acid oxidation defects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Short and branched chain specific acyl-CoA dehydrogenase that catalyzes the removal of one hydrogen from C-2 and C-3 of the fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed : 10832746, PubMed : 11013134, PubMed : 21430231, PubMed : 7698750). Among the different mitochondrial acyl-CoA dehydrogenases, acts specifically on short and branched chain acyl-CoA derivatives such as (S)-2-methylbutyryl-CoA as well as short straight chain acyl-CoAs such as butyryl-CoA (PubMed : 10832746, PubMed : 11013134, PubMed : 21430231, PubMed : 7698750). Plays an important role in the metabolism of L-isoleucine by catalyzing the dehydrogenation of 2-methylbutyryl-CoA, one of the steps of the L-isoleucine catabolic pathway (PubMed : 10832746, PubMed : 11013134). Can also act on valproyl-CoA, a metabolite of valproic acid, an antiepileptic drug (PubMed : 8660691).
See full target information Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9550 PubMed39500869

2024

Inhibition of GPX4 enhances CDK4/6 inhibitor and endocrine therapy activity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M T Herrera-Abreu,J Guan,U Khalid,J Ning,M R Costa,J Chan,Q Li,J-P Fortin,W R Wong,P Perampalam,A Biton,W Sandoval,J Vijay,M Hafner,R Cutts,G Wilson,J Frankum,T I Roumeliotis,J Alexander,O Hickman,R Brough,S Haider,J Choudhary,C J Lord,A Swain,C Metcalfe,N C Turner

Cell metabolism 29:1151-1165.e6 PubMed30661928

2019

Loss of BCAA Catabolism during Carcinogenesis Enhances mTORC1 Activity and Promotes Tumor Development and Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Russell E Ericksen,Siew Lan Lim,Eoin McDonnell,Wai Ho Shuen,Maya Vadiveloo,Phillip J White,Zhaobing Ding,Royston Kwok,Philip Lee,George K Radda,Han Chong Toh,Matthew D Hirschey,Weiping Han
View all publications
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