AB154222

Anti-SBDS antibody

Name: Anti-SBDS antibody (ab154222) | Abcam
Brand: Abcam Limited
SKU: AB154222
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SBDS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus.

View Alternative Names

CGI-97, SBDS, Ribosome maturation protein SBDS, Shwachman-Bodian-Diamond syndrome protein

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (AB154222)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (AB154222)

Immunofluorescence analysis of methanol-fixed HeLa cells, labelling SBDS with ab154222 at 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)

Immunohistochemical analysis of paraffin embedded Human H1299 xenograft tissue labelling SBDS with ab154222 at a 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)

Immunohistochemical analysis of paraffin embedded mouse duodenum tissue stained for SBDS protein at cytosol with ab154222 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Unknown

Western blot - Anti-SBDS antibody (AB154222)

12% SDS PAGE

All lanes:

Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Predicted band size: 29 kDa

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Supplier Data

Western blot - Anti-SBDS antibody (AB154222)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 29 kDa

false

Unknown

Western blot - Anti-SBDS antibody (AB154222)

12% SDS PAGE

All lanes:

Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3A5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SBDS also known as Shwachman-Bodian-Diamond syndrome protein participates in ribosome biogenesis. It has a molecular weight of approximately 29 kDa. SBDS is expressed in a variety of tissues with notable expression in the bone marrow and pancreas areas that require active ribosome synthesis. The protein works to assist in the assembly and maturation of the 60S ribosomal subunit associating closely with other ribosomal proteins and factors needed for proper cellular function.

Biological function summary

SBDS plays an essential role in the maturation of ribosomes and is part of the ribosomal large subunit assembly complex. It interacts with proteins such as EFL1 and Nucleophosmin highlighting its involvement in vital cellular processes. The protein contributes to protein synthesis machinery ensuring that ribosomal subunits are correctly formed and functional. SBDS is important for maintaining cellular homeostasis through its engagement in ribosome assembly.

Pathways

SBDS is involved in ribosome biogenesis a fundamental pathway for cell growth and division. This process is closely connected with proteins like eIF6 which cooperates with SBDS during the final stages of ribosomal subunit maturation. Additionally SBDS aligns with pathways related to cellular stress responses given its role in ensuring the integrity and function of ribosomes under conditions of cellular stress. These interactions demonstrate SBDS's importance in overarching cellular pathways that sustain life.

Mutations in the SBDS gene cause Shwachman-Diamond syndrome a rare congenital disorder that affects bone marrow function and pancreatic enzymes. The syndrome often results in neutropenia and pancreatic insufficiency manifesting through the disruption of ribosome biogenesis. Moreover research suggests SBDS might interact with proteins involved in other ribosomopathies connecting it indirectly to a spectrum of related disorders. Understanding SBDS’s role in these contexts provides insight into therapeutic targets and interventions for diseases involving ribosomal dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFL1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.

See full target information SBDS

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:197 PubMed32198344

2020

Dual regulation of p53 by the ribosome maturation factor SBDS.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Hao,Jieqiong Wang,Yajie Chen,Shanshan Wang,Mingming Cao,Hua Lu,Xiang Zhou

Cell cycle (Georgetown, Tex.) 17:2802-2818 PubMed30526248

2018

Centromere protein F (CENPF), a microtubule binding protein, modulates cancer metabolism by regulating pyruvate kinase M2 phosphorylation signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Shahid,Min Young Lee,Honit Piplani,Allen M Andres,Bo Zhou,Austin Yeon,Minjung Kim,Hyung L Kim,Jayoung Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SBDS antibody

Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (AB154222)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)

Western blot - Anti-SBDS antibody (AB154222)

Western blot - Anti-SBDS antibody (AB154222)

Western blot - Anti-SBDS antibody (AB154222)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise