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Rabbit Polyclonal SBDS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus.

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Images

Western blot - Anti-SBDS antibody (AB154222), expandable thumbnail
  • Western blot - Anti-SBDS antibody (AB154222), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (AB154222), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3A5

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Predicted
Predicted

Species
Rat, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFL1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.

Alternative names

Recommended products

Rabbit Polyclonal SBDS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3A5
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SBDS also known as Shwachman-Bodian-Diamond syndrome protein participates in ribosome biogenesis. It has a molecular weight of approximately 29 kDa. SBDS is expressed in a variety of tissues with notable expression in the bone marrow and pancreas areas that require active ribosome synthesis. The protein works to assist in the assembly and maturation of the 60S ribosomal subunit associating closely with other ribosomal proteins and factors needed for proper cellular function.

Biological function summary

SBDS plays an essential role in the maturation of ribosomes and is part of the ribosomal large subunit assembly complex. It interacts with proteins such as EFL1 and Nucleophosmin highlighting its involvement in vital cellular processes. The protein contributes to protein synthesis machinery ensuring that ribosomal subunits are correctly formed and functional. SBDS is important for maintaining cellular homeostasis through its engagement in ribosome assembly.

Pathways

SBDS is involved in ribosome biogenesis a fundamental pathway for cell growth and division. This process is closely connected with proteins like eIF6 which cooperates with SBDS during the final stages of ribosomal subunit maturation. Additionally SBDS aligns with pathways related to cellular stress responses given its role in ensuring the integrity and function of ribosomes under conditions of cellular stress. These interactions demonstrate SBDS's importance in overarching cellular pathways that sustain life.

Associated diseases and disorders

Mutations in the SBDS gene cause Shwachman-Diamond syndrome a rare congenital disorder that affects bone marrow function and pancreatic enzymes. The syndrome often results in neutropenia and pancreatic insufficiency manifesting through the disruption of ribosome biogenesis. Moreover research suggests SBDS might interact with proteins involved in other ribosomopathies connecting it indirectly to a spectrum of related disorders. Understanding SBDS’s role in these contexts provides insight into therapeutic targets and interventions for diseases involving ribosomal dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-SBDS antibody (ab154222), expandable thumbnail

    Western blot - Anti-SBDS antibody (ab154222)

    12% SDS PAGE

    All lanes: Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

    Lane 1: H1299 whole cell lysate at 30 µg

    Lane 2: HeLa whole cell lysate at 30 µg

    Lane 3: HepG2 whole cell lysate at 30 µg

    Predicted band size: 29 kDa

  • Western blot - Anti-SBDS antibody (ab154222), expandable thumbnail

    Western blot - Anti-SBDS antibody (ab154222)

    12% SDS PAGE

    All lanes: Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

    All lanes: Mouse liver whole cell lysate at 50 µg

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (ab154222), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (ab154222)

    Immunofluorescence analysis of methanol-fixed HeLa cells, labelling SBDS with ab154222 at 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (ab154222), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (ab154222)

    Immunohistochemical analysis of paraffin embedded Human H1299 xenograft tissue labelling SBDS with ab154222 at a 1/500 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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