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Rabbit Recombinant Monoclonal SBDS antibody. Suitable for WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFL1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.

Alternative names

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Rabbit Recombinant Monoclonal SBDS antibody. Suitable for WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7819

Purification technique

Affinity purification Protein A

Dissociation constant

7.4 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

SBDS plays an essential role in the maturation of ribosomes and is part of the ribosomal large subunit assembly complex. It interacts with proteins such as EFL1 and Nucleophosmin highlighting its involvement in vital cellular processes. The protein contributes to protein synthesis machinery ensuring that ribosomal subunits are correctly formed and functional. SBDS is important for maintaining cellular homeostasis through its engagement in ribosome assembly.

Activity summary

SBDS also known as Shwachman-Bodian-Diamond syndrome protein participates in ribosome biogenesis. It has a molecular weight of approximately 29 kDa. SBDS is expressed in a variety of tissues with notable expression in the bone marrow and pancreas areas that require active ribosome synthesis. The protein works to assist in the assembly and maturation of the 60S ribosomal subunit associating closely with other ribosomal proteins and factors needed for proper cellular function.

Pathways

SBDS is involved in ribosome biogenesis a fundamental pathway for cell growth and division. This process is closely connected with proteins like eIF6 which cooperates with SBDS during the final stages of ribosomal subunit maturation. Additionally SBDS aligns with pathways related to cellular stress responses given its role in ensuring the integrity and function of ribosomes under conditions of cellular stress. These interactions demonstrate SBDS's importance in overarching cellular pathways that sustain life.

Associated diseases and disorders

Mutations in the SBDS gene cause Shwachman-Diamond syndrome a rare congenital disorder that affects bone marrow function and pancreatic enzymes. The syndrome often results in neutropenia and pancreatic insufficiency manifesting through the disruption of ribosome biogenesis. Moreover research suggests SBDS might interact with proteins involved in other ribosomopathies connecting it indirectly to a spectrum of related disorders. Understanding SBDS’s role in these contexts provides insight into therapeutic targets and interventions for diseases involving ribosomal dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-SBDS antibody [EPR7819] (ab128905), expandable thumbnail

    Western blot - Anti-SBDS antibody [EPR7819] (ab128905)

    All lanes: Western blot - Anti-SBDS antibody [EPR7819] (AB128905) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: CaCo 2 cell lysate at 10 µg

    Secondary

    All lanes: Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • OI-RD Scanning - Anti-SBDS antibody [EPR7819] (ab128905), expandable thumbnail

    OI-RD Scanning - Anti-SBDS antibody [EPR7819] (ab128905)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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