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AB249935

Anti-Sbf1 antibody [EPR12566] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Sbf1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

MTMR5, SBF1, Myotubularin-related protein 5, Inactive phosphatidylinositol 3-phosphatase 5, SET-binding factor 1, Sbf1

3 Images
Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935)

This data was developed using ab177146, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Ramos cells labeling Sbf1 with ab177146 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

Western blot - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935)
  • WB

Supplier Data

Western blot - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935)

This data was developed using ab177146, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Sbf1 antibody [EPR12566] (<a href='/en-us/products/primary-antibodies/sbf1-antibody-epr12566-ab177146'>ab177146</a>) at 1/1000 dilution

Lane 1:

Raji lysate at 10 µg

Lane 2:

Ramos lysate at 10 µg

Lane 3:

Daudi lysate at 10 µg

Lane 4:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 208 kDa

false

Western blot - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935)
  • WB

Lab

Western blot - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935)

This data was developed using ab177146, the same antibody clone in a different buffer formulation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative : mouse ovary, small intestine (PMID : 11994405).

All lanes:

Western blot - Anti-Sbf1 antibody [EPR12566] (<a href='/en-us/products/primary-antibodies/sbf1-antibody-epr12566-ab177146'>ab177146</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Lane 3:

Mouse small intestine tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 208 kDa,37 kDa

false

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12566

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249935 is the carrier-free version of ab177146.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sbf1 also known as SET-binding factor 1 mechanistically acts as a pseudophosphatase due to having a phosphatase sequence motif but lacking phosphatase activity. Sbf1 with a molecular weight of approximately 206 kDa is expressed in various tissues including the brain and the immune system. It belongs to a protein family associated with cellular regulation and its pseudophosphatase domain is significant for its interactions with other proteins.
Biological function summary

Sbf1 contributes to cellular regulation and signaling pathways. It is known for its involvement in regulating cell growth and migration. Sbf1 does not act alone often forming complexes with other proteins that modulate its function. Researchers have noted its role in cellular structures involved in maintaining the cytoskeleton and intercellular connections which further implicates it in essential cellular processes.

Pathways

Sbf1 interacts within the mTOR signaling pathway a critical pathway for regulating cell growth and metabolism. It also plays a role in the PI3K/AKT pathway important for controlling various cellular processes including proliferation and survival. Through these pathways Sbf1 interacts with proteins such as mTOR and AKT contributing to maintaining cellular functions related to growth signals and nutrient sensing.

Sbf1 connects to cancer and neurological disorders. Mutations or altered expressions in Sbf1 have been observed in certain types of cancer including breast cancer where it can affect cellular proliferation and survival. Additionally its association with proteins involved in neurological processes suggests a potential link to neurodegenerative disorders. Proteins such as mTOR in cancer and AKT in neurological disorders provide direct connections to Sbf1 highlighting its role in pathways related to these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an adapter for the phosphatase MTMR2 to regulate MTMR2 catalytic activity and subcellular location (PubMed : 12668758). Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (PubMed : 20937701). May function as a guanine nucleotide exchange factor (GEF) activating RAB28 (PubMed : 20937701). Acts as a suppressor of autophagy in neurons (PubMed : 35580604). Together with its binding partner, the phosphatase MTMR2, plays a role in dephosphorylation of phosphoinositides critical for autophagy initiation and autophagosome maturation (PubMed : 35580604). Plays a role in positively regulating late-stage radial sorting of large caliber axons, a process leading to myelination by Schwann cells, possibly via regulating endosomal trafficking (By similarity). Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts (PubMed : 9537414).
See full target information SBF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong
View all publications
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Product promise

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