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Rabbit Recombinant Monoclonal Sbf1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935), expandable thumbnail
  • Western blot - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (AB249935), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Rat
Not recommended
Predicted
Not recommended
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Acts as an adapter for the phosphatase MTMR2 to regulate MTMR2 catalytic activity and subcellular location (PubMed:12668758). Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (PubMed:20937701). May function as a guanine nucleotide exchange factor (GEF) activating RAB28 (PubMed:20937701). Acts as a suppressor of autophagy in neurons (PubMed:35580604). Together with its binding partner, the phosphatase MTMR2, plays a role in dephosphorylation of phosphoinositides critical for autophagy initiation and autophagosome maturation (PubMed:35580604). Plays a role in positively regulating late-stage radial sorting of large caliber axons, a process leading to myelination by Schwann cells, possibly via regulating endosomal trafficking (By similarity). Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts (PubMed:9537414).

Alternative names

MTMR5, SBF1, Myotubularin-related protein 5, Inactive phosphatidylinositol 3-phosphatase 5, SET-binding factor 1, Sbf1

Recommended products

Rabbit Recombinant Monoclonal Sbf1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR12566
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249935 is the carrier-free version of Anti-Sbf1 antibody [EPR12566] ab177146.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Sbf1 also known as SET-binding factor 1 mechanistically acts as a pseudophosphatase due to having a phosphatase sequence motif but lacking phosphatase activity. Sbf1 with a molecular weight of approximately 206 kDa is expressed in various tissues including the brain and the immune system. It belongs to a protein family associated with cellular regulation and its pseudophosphatase domain is significant for its interactions with other proteins.

Biological function summary

Sbf1 contributes to cellular regulation and signaling pathways. It is known for its involvement in regulating cell growth and migration. Sbf1 does not act alone often forming complexes with other proteins that modulate its function. Researchers have noted its role in cellular structures involved in maintaining the cytoskeleton and intercellular connections which further implicates it in essential cellular processes.

Pathways

Sbf1 interacts within the mTOR signaling pathway a critical pathway for regulating cell growth and metabolism. It also plays a role in the PI3K/AKT pathway important for controlling various cellular processes including proliferation and survival. Through these pathways Sbf1 interacts with proteins such as mTOR and AKT contributing to maintaining cellular functions related to growth signals and nutrient sensing.

Associated diseases and disorders

Sbf1 connects to cancer and neurological disorders. Mutations or altered expressions in Sbf1 have been observed in certain types of cancer including breast cancer where it can affect cellular proliferation and survival. Additionally its association with proteins involved in neurological processes suggests a potential link to neurodegenerative disorders. Proteins such as mTOR in cancer and AKT in neurological disorders provide direct connections to Sbf1 highlighting its role in pathways related to these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (ab249935), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (ab249935)

    This data was developed using Anti-Sbf1 antibody [EPR12566] ab177146, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of permeabilized Ramos cells labeling Sbf1 with Anti-Sbf1 antibody [EPR12566] ab177146 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

  • Western blot - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (ab249935), expandable thumbnail

    Western blot - Anti-Sbf1 antibody [EPR12566] - BSA and Azide free (ab249935)

    This data was developed using Anti-Sbf1 antibody [EPR12566] ab177146, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Sbf1 antibody [EPR12566] (Anti-Sbf1 antibody [EPR12566] ab177146) at 1/1000 dilution

    Lane 1: Raji lysate at 10 µg

    Lane 2: Ramos lysate at 10 µg

    Lane 3: Daudi lysate at 10 µg

    Lane 4: Human fetal brain lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 208 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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