AB28428

Anti-SC35 antibody

BOND RX™ Validated
What is this?

(2 Reviews)

(9 Publications)

Rabbit Polyclonal SC35 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SRSF2.

View Alternative Names

SFRS2, SRSF2, Serine/arginine-rich splicing factor 2, Protein PR264, Splicing factor SC35, SC-35

2 Images

Unknown

Western blot - Anti-SC35 antibody (AB28428)

All lanes:

Western blot - Anti-SC35 antibody (ab28428)

Lane 1:

recombinant SC35 protein

Lane 2:

HeLa nuclear lysate

Predicted band size: 25 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SC35 antibody (AB28428)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SC35 antibody (AB28428)

IHC image of ab28428 staining in human seminoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28428, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SRSF2.

Q01130

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>" } } }

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Have you thought about this alternative?

AB204916

Anti-SC35 antibody [EPR12238]

2 Reviews

View product

Why is this recommended?

We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Affinity purified IgG.

Storage buffer

pH: 7.9 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SC35 also known as SRSF2 (serine/arginine-rich splicing factor 2) is an essential splicing factor with a molecular mass around 26 kDa. This protein is part of the SR protein family which are known for their roles in splicing regulation due to their serine/arginine-rich domains. SC35 predominantly localizes in the nucleus of cells where it accumulates in nuclear speckles areas rich in splicing factors. Biologists often use immunofluorescence techniques and anti-SC antibodies to visualize and study SC35's localization and function within cells.

Biological function summary

SC35 influences various aspects of mRNA processing particularly pre-mRNA splicing. The protein functions as a splicing enhancer by interacting with pre-mRNA at specific sites to assist exon inclusion during splicing. SC35 acts as part of the spliceosomal complex which is critical during the assembly and rearrangement of the spliceosome. Additionally it interacts with other splicing factors to ensure accurate and efficient splicing which is fundamental for gene expression and consequent protein synthesis.

Pathways

The SC35 protein participates in the RNA splicing pathway a significant process in mRNA maturation and gene expression regulation. This protein interacts with other SR proteins like SRSF1 and components of the spliceosomal machinery. SC35's function is also important in the alternative splicing pathway impacting the diversity of mRNA transcripts through the production of different exon combinations. By regulating alternative splicing SC35 helps produce protein diversity vital for numerous cellular processes.

Changes in SC35 expression or function have been linked to several human conditions. Notably its dysregulation can contribute to cancer development through altered splicing patterns that impact oncogene and tumor suppressor gene expression. Furthermore SC35 abnormalities are associated with spinal muscular atrophy a neurodegenerative disorder where splicing regulation plays a significant role. In these diseases SC35 may interact with other proteins such as SMN (survival of motor neuron protein) and impact their functions highlighting its relevance in maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.

See full target information SRSF2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106804 PubMed37235058

2023

Hypoxia-induced loss of SRSF2-dependent DNA methylation promotes CTCF-mediated alternative splicing of VEGFA in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Yadav,Anchala Pandey,Parik Kakani,Srinivas Abhishek Mutnuru,Atul Samaiya,Jharna Mishra,Sanjeev Shukla

OncoTargets and therapy 13:11301-11313 PubMed33177838

2020

Hsa_circ_0006916 Knockdown Represses the Development of Hepatocellular Carcinoma via Modulating miR-599/SRSF2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiang Zhu,Songhe Shen,Sen Zhao,Zhixue Wang

eLife 9: PubMed33095160

2020

SON and SRRM2 are essential for nuclear speckle formation.

Applications

Unspecified application

Species

Unspecified reactive species

İbrahim Avşar Ilik,Michal Malszycki,Anna Katharina Lübke,Claudia Schade,David Meierhofer,Tuğçe Aktaş

Molecular and cellular biology 35:3071-82 PubMed26124281

2015

SRSF2 Is Essential for Hematopoiesis, and Its Myelodysplastic Syndrome-Related Mutations Dysregulate Alternative Pre-mRNA Splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Yukiko Komeno,Yi-Jou Huang,Jinsong Qiu,Leo Lin,YiJun Xu,Yu Zhou,Liang Chen,Dora D Monterroza,Hairi Li,Russell C DeKelver,Ming Yan,Xiang-Dong Fu,Dong-Er Zhang

Retrovirology 12:29 PubMed25889056

2015

Balanced splicing at the Tat-specific HIV-1 3'ss A3 is critical for HIV-1 replication.

Applications

Unspecified application

Species

Unspecified reactive species

Steffen Erkelenz,Frank Hillebrand,Marek Widera,Stephan Theiss,Anaam Fayyaz,Daniel Degrandi,Klaus Pfeffer,Heiner Schaal

Cell reports 9:1841-1855 PubMed25482556

2014

DNA hydroxymethylation profiling reveals that WT1 mutations result in loss of TET2 function in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Raajit Rampal,Altuna Alkalin,Jozef Madzo,Aparna Vasanthakumar,Elodie Pronier,Jay Patel,Yushan Li,Jihae Ahn,Omar Abdel-Wahab,Alan Shih,Chao Lu,Patrick S Ward,Jennifer J Tsai,Todd Hricik,Valeria Tosello,Jacob E Tallman,Xinyang Zhao,Danette Daniels,Qing Dai,Luisa Ciminio,Iannis Aifantis,Chuan He,Francois Fuks,Martin S Tallman,Adolfo Ferrando,Stephen Nimer,Elisabeth Paietta,Craig B Thompson,Jonathan D Licht,Christopher E Mason,Lucy A Godley,Ari Melnick,Maria E Figueroa,Ross L Levine

European journal of human genetics : EJHG 21:317-23 PubMed22872099

2012

FTO levels affect RNA modification and the transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Tea Berulava,Matthias Ziehe,Ludger Klein-Hitpass,Emil Mladenov,Jürgen Thomale,Ulrich Rüther,Bernhard Horsthemke

Nucleic acids research 39:7077-91 PubMed21609956

2011

Compensatory signals associated with the activation of human GC 5' splice sites.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Kralovicova,Gyulin Hwang,A Charlotta Asplund,Alexander Churbanov,C I Edvard Smith,Igor Vorechovsky

Cancer research 68:4123-32 PubMed18519671

2008

Epigenetic silencing of microRNA-34b/c and B-cell translocation gene 4 is associated with CpG island methylation in colorectal cancer.

Applications

Species

Human

Minoru Toyota,Hiromu Suzuki,Yasushi Sasaki,Reo Maruyama,Kohzoh Imai,Yasuhisa Shinomura,Takashi Tokino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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sc35-antibody-epr12238-bsa-and-azide-free-ab232656

(0 reviews)

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