AB232656

Anti-SC35 antibody [EPR12238] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SC35 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SFRS2, SRSF2, Serine/arginine-rich splicing factor 2, Protein PR264, Splicing factor SC35, SC-35

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [EPR12238] - BSA and Azide free (AB232656)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [EPR12238] - BSA and Azide free (AB232656)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling SC35 with ab204916 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HEK-293 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab204916 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204916).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [EPR12238] - BSA and Azide free (AB232656)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling SC35 with ab204916 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204916).

Supplier Data

Immunoprecipitation - Anti-SC35 antibody [EPR12238] - BSA and Azide free (AB232656)

SC35 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab204916 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab204916 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab204916 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab204916 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204916).

All lanes:

Immunoprecipitation - Anti-SC35 antibody [EPR12238] (<a href='/en-us/products/primary-antibodies/sc35-antibody-epr12238-ab204916'>ab204916</a>)

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12238

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab232656 is the carrier-free version of ab204916.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SC35 also known as SRSF2 (serine/arginine-rich splicing factor 2) is an essential splicing factor with a molecular mass around 26 kDa. This protein is part of the SR protein family which are known for their roles in splicing regulation due to their serine/arginine-rich domains. SC35 predominantly localizes in the nucleus of cells where it accumulates in nuclear speckles areas rich in splicing factors. Biologists often use immunofluorescence techniques and anti-SC antibodies to visualize and study SC35's localization and function within cells.

Biological function summary

SC35 influences various aspects of mRNA processing particularly pre-mRNA splicing. The protein functions as a splicing enhancer by interacting with pre-mRNA at specific sites to assist exon inclusion during splicing. SC35 acts as part of the spliceosomal complex which is critical during the assembly and rearrangement of the spliceosome. Additionally it interacts with other splicing factors to ensure accurate and efficient splicing which is fundamental for gene expression and consequent protein synthesis.

Pathways

The SC35 protein participates in the RNA splicing pathway a significant process in mRNA maturation and gene expression regulation. This protein interacts with other SR proteins like SRSF1 and components of the spliceosomal machinery. SC35's function is also important in the alternative splicing pathway impacting the diversity of mRNA transcripts through the production of different exon combinations. By regulating alternative splicing SC35 helps produce protein diversity vital for numerous cellular processes.

Changes in SC35 expression or function have been linked to several human conditions. Notably its dysregulation can contribute to cancer development through altered splicing patterns that impact oncogene and tumor suppressor gene expression. Furthermore SC35 abnormalities are associated with spinal muscular atrophy a neurodegenerative disorder where splicing regulation plays a significant role. In these diseases SC35 may interact with other proteins such as SMN (survival of motor neuron protein) and impact their functions highlighting its relevance in maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.

See full target information SRSF2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39273021

2024

Cell-Sonar, a Novel Method for Intracellular Tracking of Secretory Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Thomas Seeger,Franz Worek,Simone Rothmiller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SC35 antibody [EPR12238] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [EPR12238] - BSA and Azide free (AB232656)

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [EPR12238] - BSA and Azide free (AB232656)

Immunoprecipitation - Anti-SC35 antibody [EPR12238] - BSA and Azide free (AB232656)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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