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Mouse Monoclonal SC35 antibody. Nuclear Speckle marker. Suitable for ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 138 publications.
Alternative names=Serine/arginine-rich splicing factor 2, Protein PR264, Splicing factor SC35, SC-35, SRSF2, SFRS2
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 93% PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Not recommended | Tested |
| Mouse | Not recommended | Tested |
| Rat | Not recommended | Tested |
| Drosophila melanogaster | Not recommended | Predicted |
| Newt | Not recommended | Predicted |
| Rhesus monkey | Not recommended | Predicted |
| Xenopus laevis | Not recommended | Predicted |
Images
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Lysis buffer: 6.0% (w/v) SDS, 0.14 mol Tris (pH 6.8), 22.4% (v/v) glycerol, protease inhibitor cocktail. Centrifuge at 14 000 g for 15 min at 48C. Block non-specific binding sites with 5% (w/v) skimmed milk powder in TBS with 0.1% (v/v) Tween 20 for 1 h. Incubate blots with primary antibody (diluted in 5% (w/v) skimmed milk powder in TBS with 0.1% (v/v) Tween 20) for 1 h at room temperature. |
Species Rat | Dilution info - | Notes Lysis buffer: 6.0% (w/v) SDS, 0.14 mol Tris (pH 6.8), 22.4% (v/v) glycerol, protease inhibitor cocktail. Centrifuge at 14 000 g for 15 min at 48C. Block non-specific binding sites with 5% (w/v) skimmed milk powder in TBS with 0.1% (v/v) Tween 20 for 1 h. Incubate blots with primary antibody (diluted in 5% (w/v) skimmed milk powder in TBS with 0.1% (v/v) Tween 20) for 1 h at room temperature. |
Species Human | Dilution info - | Notes Lysis buffer: 6.0% (w/v) SDS, 0.14 mol Tris (pH 6.8), 22.4% (v/v) glycerol, protease inhibitor cocktail. Centrifuge at 14 000 g for 15 min at 48C. Block non-specific binding sites with 5% (w/v) skimmed milk powder in TBS with 0.1% (v/v) Tween 20 for 1 h. Incubate blots with primary antibody (diluted in 5% (w/v) skimmed milk powder in TBS with 0.1% (v/v) Tween 20) for 1 h at room temperature. |
Species Xenopus laevis | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info - | Notes - |
Species Rhesus monkey | Dilution info - | Notes - |
Species Newt | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Rat | Dilution info 5 µg/mL | Notes - |
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Drosophila melanogaster, Rhesus monkey, Newt | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal SC35 antibody. Nuclear Speckle marker. Suitable for ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 138 publications.
Alternative names=Serine/arginine-rich splicing factor 2, Protein PR264, Splicing factor SC35, SC-35, SRSF2, SFRS2
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 93% PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Clone number
SC-35
- Purification technique
Affinity purification Protein G
- Specificity
This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. Recent data suggests this clone may cross-react with additional proteins within the spliceosome complex (PMID: 33095160)
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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