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AB221764

Anti-SC5DL antibody

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(2 Publications)

Rabbit Polyclonal SC5DL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SC5D aa 50-100.

View Alternative Names

SC5DL, SC5D, Lathosterol oxidase, C-5 sterol desaturase, Delta(7)-sterol 5-desaturase, Delta(7)-sterol C5(6)-desaturase, Lathosterol 5-desaturase, Sterol-C5-desaturase

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SC5DL antibody (AB221764)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SC5DL antibody (AB221764)

PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for SC5DL (green) using ab221764 at 4 μg/ml in ICC/IF.

Western blot - Anti-SC5DL antibody (AB221764)
  • WB

Supplier Data

Western blot - Anti-SC5DL antibody (AB221764)

All lanes:

Western blot - Anti-SC5DL antibody (ab221764) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SC5D aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O75845

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SC5DL also known as sterol-C5-desaturase or MIG12 is an enzyme important in cholesterol biosynthesis. Weighing approximately 39 kDa SC5DL catalyzes the conversion of lathosterol to 7-dehydrocholesterol by introducing a C5-C6 double bond in the sterol ring. This enzyme predominantly expresses in the liver but is also found in lesser amounts in other tissues such as the brain and skin.
Biological function summary

This enzyme plays a role in the synthesis of cholesterol an essential lipid molecule for membrane structure and function. SC5DL operates within the endoplasmic reticulum often as part of a complex with other enzymes in the cholesterol synthesis pathway. This activity serves not just in producing cholesterol for cell membrane integrity but also generates precursor molecules for steroid hormone production and vitamin D synthesis.

Pathways

SC5DL is an integral part of the mevalonate pathway a critical metabolic pathway for cholesterol synthesis. This pathway involves multiple enzymes and SC5DL works closely with others like HMG-CoA reductase. SC5DL also participates in the Kandutsch-Russell pathway interacting with proteins such as DHCR7 to regulate the flow of sterol intermediates and maintain cholesterol homeostasis.

Alterations in SC5DL activity can impact cholesterol levels and are linked to conditions such as congenital disorders of cholesterol biosynthesis. Mutations or dysfunctions in SC5DL associate with lathosterolosis causing developmental abnormalities due to impaired cholesterol synthesis. Additionally changes in expression of SC5DL and its interaction with proteins like DHCR7 have been studied for their implications in metabolic disorders like Smith-Lemli-Opitz syndrome.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the penultimate step of the biosynthesis of cholesterol, the dehydrogenation of lathosterol into 7-dehydrocholesterol (7-DHC). Cholesterol is the major sterol component in mammalian membranes and a precursor for bile acid and steroid hormone synthesis (PubMed : 10786622, PubMed : 38297129). In addition to its essential role in cholesterol biosynthesis, it also indirectly regulates ferroptosis through the production of 7-DHC. By diverting the spread of damage caused by peroxyl radicals from the phospholipid components to its sterol nucleus, 7-DHC prevents this form of cell death (PubMed : 38297129, PubMed : 38297130).
See full target information SC5D
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110651 PubMed39262789

2024

Knockouts of , , or from cholesterol synthesis reveal pathways modulated by sterol intermediates.

Applications

Unspecified application

Species

Unspecified reactive species

Cene Skubic,Hana Trček,Petra Nassib,Tinkara Kreft,Andrew Walakira,Katka Pohar,Sara Petek,Tadeja Režen,Alojz Ihan,Damjana Rozman

Journal of molecular histology 50:63-73 PubMed30535733

2018

Subcellular localization of sterol biosynthesis enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Katalin Koczok,Channabasavaiah B Gurumurthy,István Balogh,Zeljka Korade,Károly Mirnics
View all publications
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Product promise

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