AB227922

Anti-SCA2 antibody

Name: Anti-SCA2 antibody (ab227922) | Abcam
Brand: Abcam Limited
SKU: AB227922
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal SCA2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATXN2.

View Alternative Names

ATX2, SCA2, TNRC13, ATXN2, Ataxin-2, Spinocerebellar ataxia type 2 protein, Trinucleotide repeat-containing gene 13 protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody (AB227922)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody (AB227922)

Paraffin-embedded mouse brain tissue stained for SCA2 using ab227922 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SCA2 antibody (AB227922)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SCA2 antibody (ab227922) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 140 kDa

false

Supplier Data

Western blot - Anti-SCA2 antibody (AB227922)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-SCA2 antibody (ab227922) at 1/3000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

ATXN2 shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ATXN2. The exact immunogen used to generate this antibody is proprietary information.

Q99700

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCA2 also known as Spinocerebellar Ataxia Type 2 refers to a protein primarily affected by mutations in the ATXN2 gene. It weighs around 150 kDa. SCA2 expresses predominantly in the brain with significant presence in Purkinje cells of the cerebellum as well as regions like the thalamus and hippocampus. It functions as an essential component within neuron-specific structures and may associate with RNA processing events.

Biological function summary

SCA2 shows significant interactions within the neuronal RNA processing machinery. This protein is part of a large complex involved in regulating RNA stability and translation. Its role contributes significantly to maintaining neuron function and viability affecting synaptic signaling and plasticity which are critical for cognitive processes and motor control. SCA2 influences neural communication through modulation and degradation of messenger RNAs.

Pathways

SCA2 plays a role in the mRNA metabolic process related cellular pathways. It interacts in pathways like RNA transport and degradation linking it functionally to proteins such as TDP-43 and FUS which also have prominent roles in RNA processing. These associations support communication across neurons and ensure accurate protein synthesis necessary for neuronal health and function.

Malfunction or mutation in SCA2 relates directly to spinocerebellar ataxia type 2 a degenerative disorder affecting movement. The imbalance in related pathways often leads to pathological changes contributing to dysfunctions in Ataxin-2 often seen alongside interactions with proteins like Huntingtin which elucidate its involvement in neurological diseases. Such connections imply a substantial impact on neuronal survival and disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane.

See full target information ATXN2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:417 PubMed40883266

2025

METTL3-mediated m6A modification of circCDKAL1 regulates macrophage M1 polarization and nasal epithelial cell barrier function in allergic rhinitis through IGF2BP2/JARID2/HMGB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabin Zhan,Dan Luo,Yunlong Fu,Yu Zhou,Rui Li,Xin Wei

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody (AB227922)

Western blot - Anti-SCA2 antibody (AB227922)

Western blot - Anti-SCA2 antibody (AB227922)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Biochemicals

Primary Antibodies

Product promise