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AB124688

Anti-SCAI antibody [EPR4128]

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(7 Publications)

Rabbit Recombinant Monoclonal SCAI antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

C9orf126, SCAI, Protein SCAI, Suppressor of cancer cell invasion protein

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (AB124688)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (AB124688)

Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (AB124688)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (AB124688)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] (AB124688)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] (AB124688)

Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by intracellular flow cytometry (red) compared to a nonspecific negative control antibody (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (AB124688)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (AB124688)

Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] (AB124688)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] (AB124688)

Overlay histogram showingSH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (AB124688)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (AB124688)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)
  • WB

Unknown

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution

Lane 1:

Human Thymus lysate at 10 µg

Lane 2:

Human Thyroid lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 11 kDa,70 kDa

false

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)
  • WB

Lab

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution

All lanes:

Human thyroid tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 66 kDa

false

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)
  • WB

Lab

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 66 kDa

false

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)
  • WB

Lab

Western blot - Anti-SCAI antibody [EPR4128] (AB124688)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 66 kDa

false

OI-RD Scanning - Anti-SCAI antibody [EPR4128] (AB124688)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SCAI antibody [EPR4128] (AB124688)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SCAI antibody [EPR4128] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4128

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p><strong>For unpurified, use 1/5.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p><strong>For unpurified, use 1/200.</strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/400", "ICCIF-species-notes": "<p><strong>For unpurified, use 1/50.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/160", "FlowCytIntra-species-notes": "<p>For unpurified, use 1/20. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p><strong>For unpurified, use 1/200.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p><strong>For unpurified, use 1/200.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCAI also known as Suppressor of Cancer Cell Invasion is a protein involved in the regulation of cell motility. It has a molecular weight of approximately 96 kDa. SCAI is expressed in various human tissues with a notable presence in the heart and skeletal muscle. This protein plays an important role in modulating the dynamic interactions between the cellular cytoskeleton and regulatory proteins directly affecting the cell's ability to move and invade surrounding tissues.
Biological function summary

SCAI influences cell behavior through its interaction with components of the actin cytoskeleton. It serves as an essential part of the complex that suppresses the invasive behavior of cancer cells. By binding with transcription factors like MRTF and influencing their activity SCAI regulates gene expression linked to cellular movement. This regulation helps maintain the structural integrity of tissues and prevents abnormal cellular invasiveness.

Pathways

Suppression of invasive signaling connects SCAI to vital molecular mechanisms. It significantly interfaces with the SRF (serum response factor) signaling pathway directly affecting processes involving actin dynamics and transcriptional regulation. SCAI also interacts with proteins such as MRTF and G-actin playing an important role in these pathways by inhibiting excessive cellular movement maintaining normal cell function.

Links between SCAI and aggressive cancer types such as breast cancer are well-established. Changes in SCAI expression are associated with increased cancer cell invasiveness and metastasis. The protein influences disease processes partly through its interaction with MRTF a critical player in oncogenic pathways. By modulating MRTF's activity SCAI potentially affects cancer progression highlighting its importance in disease regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tumor suppressor which functions to suppress MRTFA-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.
See full target information SCAI
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:220 PubMed39563244

2024

BMSC-derived exosomes promote osteoporosis alleviation via M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbin Zhang,Jing Bai,Bin Xiao,Chunyan Li

The Journal of clinical investigation 134: PubMed39361422

2024

Transcription factor KROX20 marks epithelial stem cell ancestors for hair follicle formation.

Applications

Unspecified application

Species

Unspecified reactive species

Elnaz Ghotbi,Edem Tchegnon,Zhiguo Chen,Stephen Li,Tracey Shipman,Yong Wang,Jenny Raman,Yumeng Zhang,Renee M McKay,Chung-Ping Liao,Lu Q Le

Molecular cell 81:4440-4456.e7 PubMed34597596

2021

The Protexin complex counters resection on stalled forks to promote homologous recombination and crosslink repair.

Applications

Unspecified application

Species

Unspecified reactive species

Richard O Adeyemi,Nicholas A Willis,Andrew E H Elia,Connor Clairmont,Shibo Li,Xiaohua Wu,Alan D D'Andrea,Ralph Scully,Stephen J Elledge

Cancer management and research 12:7501-7514 PubMed32943917

2020

Exosomal CircGDI2 Suppresses Oral Squamous Cell Carcinoma Progression Through the Regulation of MiR-424-5p/SCAI Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Kaiqi Tang,Lizhu Chen,Meng Du,Zhi Qu

Molecular therapy. Nucleic acids 18:24-33 PubMed31479922

2019

Circular RNA Cdr1as Upregulates SCAI to Suppress Cisplatin Resistance in Ovarian Cancer via miR-1270 Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Zhao,Mei Ji,Qianqing Wang,Nannan He,Yue Li

BMC nephrology 18:320 PubMed29065889

2017

Injury induced expression of caveolar proteins in human kidney tubules - role of megakaryoblastic leukemia 1.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof M Krawczyk,Jennifer Hansson,Helén Nilsson,Katarzyna K Krawczyk,Karl Swärd,Martin E Johansson

Molecular medicine reports 15:1631-1637 PubMed28260055

2017

miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity.

Applications

ICC/IF

Species

Human

Yonggen Zou,Yuanshuai Huang,Jiexiang Yang,Jian Wu,Cheng Luo
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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