Anti-SCAI antibody [EPR4128] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by intracellular flow cytometry (red) compared to a nonspecific negative control antibody (green).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Overlay histogram showing SH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in 160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

• WB

Unknown

Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (<a href='/en-us/products/primary-antibodies/scai-antibody-epr4128-ab124688'>ab124688</a>) at 1/1000 dilution

Lane 1:

Human Thymus lysate at 10 µg

Lane 2:

Human Thyroid lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 11 kDa,70 kDa

false

• WB

Lab

Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (<a href='/en-us/products/primary-antibodies/scai-antibody-epr4128-ab124688'>ab124688</a>) at 1/1600 dilution

All lanes:

Human thyroid tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 66 kDa

false

• WB

Lab

Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (<a href='/en-us/products/primary-antibodies/scai-antibody-epr4128-ab124688'>ab124688</a>) at 1/1600 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 66 kDa

false

• WB

Lab

Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

This data was developed using ab124688, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SCAI antibody [EPR4128] (<a href='/en-us/products/primary-antibodies/scai-antibody-epr4128-ab124688'>ab124688</a>) at 1/200 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 66 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.