Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)

Rabbit Recombinant Monoclonal SCAI antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] - BSA and Azide free (AB247960), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Tested	Expected
Rat	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information SCAI

Function

Tumor suppressor which functions to suppress MRTFA-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.

Alternative names

C9orf126, SCAI, Protein SCAI, Suppressor of cancer cell invasion protein

Recommended products

Rabbit Recombinant Monoclonal SCAI antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR4128
Purification technique: Affinity purification Protein A
Dissociation constant: 5.8 x 10^-12 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab247960 is the carrier-free version of Anti-SCAI antibody [EPR4128] ab124688.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SCAI also known as Suppressor of Cancer Cell Invasion is a protein involved in the regulation of cell motility. It has a molecular weight of approximately 96 kDa. SCAI is expressed in various human tissues with a notable presence in the heart and skeletal muscle. This protein plays an important role in modulating the dynamic interactions between the cellular cytoskeleton and regulatory proteins directly affecting the cell's ability to move and invade surrounding tissues.

Biological function summary

SCAI influences cell behavior through its interaction with components of the actin cytoskeleton. It serves as an essential part of the complex that suppresses the invasive behavior of cancer cells. By binding with transcription factors like MRTF and influencing their activity SCAI regulates gene expression linked to cellular movement. This regulation helps maintain the structural integrity of tissues and prevents abnormal cellular invasiveness.

Pathways

Suppression of invasive signaling connects SCAI to vital molecular mechanisms. It significantly interfaces with the SRF (serum response factor) signaling pathway directly affecting processes involving actin dynamics and transcriptional regulation. SCAI also interacts with proteins such as MRTF and G-actin playing an important role in these pathways by inhibiting excessive cellular movement maintaining normal cell function.

Associated diseases and disorders

Links between SCAI and aggressive cancer types such as breast cancer are well-established. Changes in SCAI expression are associated with increased cancer cell invasiveness and metastasis. The protein influences disease processes partly through its interaction with MRTF a critical player in oncogenic pathways. By modulating MRTF's activity SCAI potentially affects cancer progression highlighting its importance in disease regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified Anti-SCAI antibody [EPR4128] ab124688 at a dilution of 1/400. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SCAI antibody [EPR4128] (Anti-SCAI antibody [EPR4128] ab124688) at 1/1600 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa
Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SCAI antibody [EPR4128] (Anti-SCAI antibody [EPR4128] ab124688) at 1/1600 dilution
All lanes: Human thyroid tissue lysate at 20 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human brain with purified Anti-SCAI antibody [EPR4128] ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.
Overlay histogram showing SH-SY5Y cells fixed in 2% PFA and stained with purified Anti-SCAI antibody [EPR4128] ab124688 at a dilution of 1 in 160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SCAI antibody [EPR4128] (Anti-SCAI antibody [EPR4128] ab124688) at 1/1000 dilution
Lane 1: Human Thymus lysate at 10 µg
Lane 2: Human Thyroid lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Secondary
All lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 11 kDa, 70 kDa
Western blot - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SCAI antibody [EPR4128] (Anti-SCAI antibody [EPR4128] ab124688) at 1/200 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human brain with unpurified Anti-SCAI antibody [EPR4128] ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified Anti-SCAI antibody [EPR4128] ab124688 at a dilution of 1/50. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
This data was developed using Anti-SCAI antibody [EPR4128] ab124688, the same antibody clone in a different buffer formulation.
Unpurified Anti-SCAI antibody [EPR4128] ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by intracellular flow cytometry (red) compared to a nonspecific negative control antibody (green).
OI-RD Scanning - Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.