AB190103

Anti-SCAP antibody

Name: Anti-SCAP antibody (ab190103) | Abcam
Brand: Abcam Limited
SKU: AB190103
Price: 630 USD
Availability: InStock

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(15 Publications)

Rabbit Polyclonal SCAP antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human SCAP aa 600 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0199, PSEC0227, SCAP, Sterol regulatory element-binding protein cleavage-activating protein, SREBP cleavage-activating protein

5 Images

Flow Cytometry (Intracellular) - Anti-SCAP antibody (AB190103)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SCAP antibody (AB190103)

Overlay histogram showing K562 cells stained with ab190103 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ab190103, 1 : 25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAP antibody (AB190103)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAP antibody (AB190103)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling SCAP with ab190103 at 5 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-SCAP antibody (AB190103)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SCAP antibody (AB190103)

Immunofluorescent analysis of HeLa cells (4% PFA-fixed, 20min; Triton X-100 permeabilized, 0.2%, 30 min) labeling SCAP with ab190103 at 1/100 dilution for 2h RT. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAP antibody (AB190103)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SCAP with ab190103 at 5μg/ml dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

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Western blot - Anti-SCAP antibody (AB190103)

All lanes:

Western blot - Anti-SCAP antibody (ab190103) at 1/1000 dilution

All lanes:

K562 cell lysate at 35 µg

Predicted band size: 140 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Human SCAP aa 600 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q12770

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10-50 µg/mg of lysate", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCAP also known as SREBP cleavage-activating protein is an important player in lipid homeostasis. SCAP has a molecular mass of approximately 150 kDa. It locates in the endoplasmic reticulum and Golgi apparatus within cells. The protein facilitates the movement of sterol regulatory element-binding proteins (SREBPs) from the endoplasmic reticulum to the Golgi. This action activates SREBPs by enabling their cleavage which is necessary for cholesterol and lipid synthesis regulation.

Biological function summary

SCAP acts as a cholesterol sensor when paired with SREBPs in a complex. The SCAP-SREBP complex remains inactive within the endoplasmic reticulum at high cholesterol concentrations. When cholesterol levels decrease SCAP aids in the transport of SREBPs to the Golgi apparatus. This transport triggers proteolytic activation of SREBPs which then migrate to the nucleus to promote gene expression for lipid metabolism.

Pathways

SCAP is instrumental in the cholesterol biosynthesis and lipid metabolic pathways. The protein interacts closely with SREBPs in these pathways to regulate lipid balance within cells. In particular SCAP's action is linked with the insulin signaling pathway aiding in the coordination of growth factor-mediated lipid synthesis. This highlights its importance in the control of lipid-related gene transcription together with proteins like Insig which anchor the SCAP-SREBP complex under high cholesterol levels.

SCAP plays a role in metabolic syndrome and cardiovascular diseases. Dysregulation of SCAP can lead to abnormal cholesterol levels contributing to atherosclerosis and other lipid disorders. The function of SCAP connects to proteins like HMG-CoA reductase in these conditions as it co-regulates the synthesis of cholesterol. Understanding SCAP's role in these diseases may open new therapeutic strategies for managing cholesterol-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Escort protein required for cholesterol as well as lipid homeostasis (By similarity). Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed : 26311497). At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum (By similarity). Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi : in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (PubMed : 26311497). Binds cholesterol via its SSD domain (By similarity).

See full target information SCAP

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 45:1038-1071 PubMed40443016

2025

Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghan Zhu,Yixiao Xiong,Yuxin Zhang,Huifang Liang,Kun Cheng,Yuanxiang Lu,Guangzhen Cai,Yang Wu,Yunhui Fan,Xiaoping Chen,Hong Zhu,Zeyang Ding,Wanguang Zhang

Cellular and molecular life sciences : CMLS 81:289 PubMed38970696

2024

Human cytomegalovirus infection impairs neural differentiation via repressing sterol regulatory element binding protein 2-mediated cholesterol biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Li,Jingxuan Sun,Mingyi Xu,Lei Yang,Ning Yang,Jingui Deng,Yanping Ma,Ying Qi,Zhongyang Liu,Qiang Ruan,Yao Liu,Yujing Huang

Journal of dairy science 106:5626-5635 PubMed37291038

2023

Low abundance of insulin-induced gene 1 contributes to SREBP-1c processing and hepatic steatosis in dairy cows with severe fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Zhu,Lin Lei,Xinghui Wang,Qianming Jiang,Juan J Loor,Fanrong Kong,Linfang Chen,Jinxia Li,Chenchen Zhao,Menglin Liu,Guowen Liu,Xinwei Li

Respiratory research 23:338 PubMed36496421

2022

D-Limonene inhibits the occurrence and progression of LUAD through suppressing lipid droplet accumulation induced by PM exposure in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Tengteng Zhu,Yangyang Li,Tienan Feng,Yuqing Yang,Kai Zhang,Jing Gao,Xiaowei Quan,Ying Qian,Herbert Yu,Biyun Qian

Cell reports 41:111738 PubMed36450259

2022

Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchun Wang,Yuzhen Wang,Lu Ding,Xiaolei Ren,Bo Wang,Liyuan Wang,Songbo Zhao,Xuetian Yue,Zhuanchang Wu,Chunyang Li,Xiaohong Liang,Chunhong Ma,Lifen Gao

International journal of molecular sciences 23: PubMed36293007

2022

LncGSAR Controls Ovarian Granulosa Cell Steroidogenesis via Sponging MiR-125b to Activate SCAP/SREBP Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wang,Yunxia Guo,Chunhui Duan,Junjie Li,Shoukun Ji,Huihui Yan,Yueqin Liu,Yingjie Zhang

International journal of molecular sciences 23: PubMed35806291

2022

Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tung-Yu Tiong,Pei-Wei Weng,Chun-Hua Wang,Syahru Agung Setiawan,Vijesh Kumar Yadav,Narpati Wesa Pikatan,Iat-Hang Fong,Chi-Tai Yeh,Chia-Hung Hsu,Kuang-Tai Kuo

Folia histochemica et cytobiologica 59:134-143 PubMed34151999

2021

Golgi a-mannosidase II mediates the formation of vascular smooth muscle foam cells under inflammatory stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kelan Zha,Qiang Ye

Food science & nutrition 9:209-216 PubMed33473285

2021

Curcumin inhibits the proteolytic process of SREBP-2 by first inhibiting the expression of S1P rather than directly inhibiting SREBP-2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yongnan Li,Shuodong Wu

International urology and nephrology : PubMed33389513

2021

Interference of S100A16 suppresses lipid accumulation and inflammation in high glucose-induced HK-2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lanlan Liu,Shan Lan

View all publications

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Product promise

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Anti-SCAP antibody

Flow Cytometry (Intracellular) - Anti-SCAP antibody (AB190103)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAP antibody (AB190103)

Immunocytochemistry/ Immunofluorescence - Anti-SCAP antibody (AB190103)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAP antibody (AB190103)

Western blot - Anti-SCAP antibody (AB190103)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise