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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal SCAP antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SCAP aa 600-650 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted | Predicted |
Chinese hamster | Predicted | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig, Chinese hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig, Chinese hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig, Chinese hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10.00000 - 1/50.00000 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig, Chinese hamster | Dilution info - | Notes - |
Escort protein required for cholesterol as well as lipid homeostasis (By similarity). Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (By similarity). At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum (By similarity). Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity). Binds cholesterol via its SSD domain (By similarity).
Sterol regulatory element-binding protein cleavage-activating protein, SCAP, SREBP cleavage-activating protein, KIAA0199, SCAP, PSEC0227
Rabbit Polyclonal SCAP antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SCAP aa 600-650 conjugated to Keyhole Limpet Haemocyanin.
Sterol regulatory element-binding protein cleavage-activating protein, SCAP, SREBP cleavage-activating protein, KIAA0199, SCAP, PSEC0227
IgG
Rabbit
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
SCAP acts as a cholesterol sensor when paired with SREBPs in a complex. The SCAP-SREBP complex remains inactive within the endoplasmic reticulum at high cholesterol concentrations. When cholesterol levels decrease SCAP aids in the transport of SREBPs to the Golgi apparatus. This transport triggers proteolytic activation of SREBPs which then migrate to the nucleus to promote gene expression for lipid metabolism.
SCAP also known as SREBP cleavage-activating protein is an important player in lipid homeostasis. SCAP has a molecular mass of approximately 150 kDa. It locates in the endoplasmic reticulum and Golgi apparatus within cells. The protein facilitates the movement of sterol regulatory element-binding proteins (SREBPs) from the endoplasmic reticulum to the Golgi. This action activates SREBPs by enabling their cleavage which is necessary for cholesterol and lipid synthesis regulation.
SCAP is instrumental in the cholesterol biosynthesis and lipid metabolic pathways. The protein interacts closely with SREBPs in these pathways to regulate lipid balance within cells. In particular SCAP's action is linked with the insulin signaling pathway aiding in the coordination of growth factor-mediated lipid synthesis. This highlights its importance in the control of lipid-related gene transcription together with proteins like Insig which anchor the SCAP-SREBP complex under high cholesterol levels.
SCAP plays a role in metabolic syndrome and cardiovascular diseases. Dysregulation of SCAP can lead to abnormal cholesterol levels contributing to atherosclerosis and other lipid disorders. The function of SCAP connects to proteins like HMG-CoA reductase in these conditions as it co-regulates the synthesis of cholesterol. Understanding SCAP's role in these diseases may open new therapeutic strategies for managing cholesterol-related disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-SCAP antibody (AB190103) at 1/1000 dilution
All lanes: K562 cell lysate at 35 µg
Predicted band size: 140 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling SCAP with ab190103 at 5 μg/ml.
Immunofluorescent analysis of HeLa cells (4% PFA-fixed, 20min; Triton X-100 permeabilized, 0.2%, 30 min) labeling SCAP with ab190103 at 1/100 dilution for 2h RT. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SCAP with ab190103 at 5μg/ml dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Intracellular flow cytometric analysis of K562 cells labeling SCAP with ab190103 at 1/10 dilution (lower image) compared with a negative control (upper image). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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