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Rabbit Recombinant Monoclonal SCAP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (AB308061), expandable thumbnail
  • Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (AB308061), expandable thumbnail
  • Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (AB308061), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Escort protein required for cholesterol as well as lipid homeostasis (By similarity). Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:26311497). At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum (By similarity). Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (PubMed:26311497). Binds cholesterol via its SSD domain (By similarity).

Alternative names

KIAA0199, PSEC0227, SCAP, Sterol regulatory element-binding protein cleavage-activating protein, SREBP cleavage-activating protein

Recommended products

Rabbit Recombinant Monoclonal SCAP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26242-60
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SCAP also known as SREBP cleavage-activating protein is an important player in lipid homeostasis. SCAP has a molecular mass of approximately 150 kDa. It locates in the endoplasmic reticulum and Golgi apparatus within cells. The protein facilitates the movement of sterol regulatory element-binding proteins (SREBPs) from the endoplasmic reticulum to the Golgi. This action activates SREBPs by enabling their cleavage which is necessary for cholesterol and lipid synthesis regulation.

Biological function summary

SCAP acts as a cholesterol sensor when paired with SREBPs in a complex. The SCAP-SREBP complex remains inactive within the endoplasmic reticulum at high cholesterol concentrations. When cholesterol levels decrease SCAP aids in the transport of SREBPs to the Golgi apparatus. This transport triggers proteolytic activation of SREBPs which then migrate to the nucleus to promote gene expression for lipid metabolism.

Pathways

SCAP is instrumental in the cholesterol biosynthesis and lipid metabolic pathways. The protein interacts closely with SREBPs in these pathways to regulate lipid balance within cells. In particular SCAP's action is linked with the insulin signaling pathway aiding in the coordination of growth factor-mediated lipid synthesis. This highlights its importance in the control of lipid-related gene transcription together with proteins like Insig which anchor the SCAP-SREBP complex under high cholesterol levels.

Associated diseases and disorders

SCAP plays a role in metabolic syndrome and cardiovascular diseases. Dysregulation of SCAP can lead to abnormal cholesterol levels contributing to atherosclerosis and other lipid disorders. The function of SCAP connects to proteins like HMG-CoA reductase in these conditions as it co-regulates the synthesis of cholesterol. Understanding SCAP's role in these diseases may open new therapeutic strategies for managing cholesterol-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (ab308061), expandable thumbnail

    Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (ab308061)

    This data was developed using Anti-SCAP antibody [EPR26242-60] ab308060, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In lanes 1-2, the lysates were stored at -80°C prior to Western Blotting.

    The bands beneath the target band (150 kDa) are likey to be degradation products.

    In lanes 3-4, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Samples are non-boiled as boiling may cause protein aggregation.

    Exposure time: 81 seconds.

    All lanes: Western blot - Anti-SCAP antibody [EPR26242-60] (Anti-SCAP antibody [EPR26242-60] ab308060) at 1/1000 dilution

    Lane 1: NIH/3T3 whole cell lysate at 20 µg

    Lane 2: PC-12 whole cell lysate at 20 µg

    Lane 3: NIH/3T3 fresh whole cell lysate at 20 µg

    Lane 4: PC-12 fresh whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 81s

  • Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (ab308061), expandable thumbnail

    Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (ab308061)

    This data was developed using Anti-SCAP antibody [EPR26242-60] ab308060, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.

    Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-SCAP antibody [EPR26242-60] (Anti-SCAP antibody [EPR26242-60] ab308060) at 1/1000 dilution

    Lane 1: DLD-1 whole cell lysate at 20 µg

    Lane 2: LNCaP whole cell lysate at 20 µg

    Lane 3: HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 4: HeLa transfected with siRNA specifically targeting Human SCAP whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 48s

  • Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (ab308061), expandable thumbnail

    Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (ab308061)

    This data was developed using Anti-SCAP antibody [EPR26242-60] ab308060, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    Samples are non-boiled as boiling may cause protein aggregation.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-SCAP antibody [EPR26242-60] (Anti-SCAP antibody [EPR26242-60] ab308060) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate

    Lane 2: Mouse spleen tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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