Anti-SCAP antibody [EPR26242-60] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (AB308061)

This data was developed using ab308060, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Samples are non-boiled as boiling may cause protein aggregates. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 48 seconds.

All lanes:

Western blot - Anti-SCAP antibody [EPR26242-60] (<a href='/en-us/products/primary-antibodies/scap-antibody-epr26242-60-ab308060'>ab308060</a>) at 1/1000 dilution

Lane 1:

DLD-1 whole cell lysate at 20 µg

Lane 2:

LNCaP whole cell lysate at 20 µg

Lane 3:

HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 4:

HeLa transfected with siRNA specifically targeting Human SCAP whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (AB308061)

This data was developed using ab308060, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation. Samples are non-boiled as boiling may cause protein aggregation. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-SCAP antibody [EPR26242-60] (<a href='/en-us/products/primary-antibodies/scap-antibody-epr26242-60-ab308060'>ab308060</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate

Lane 2:

Mouse spleen tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-SCAP antibody [EPR26242-60] - BSA and Azide free (AB308061)

This data was developed using ab308060, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. In lanes 1-2, the lysates were stored at -80°C prior to Western Blotting. The bands beneath the target band (150 kDa) are likey to be degradation products. In lanes 3-4, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Samples are non-boiled as boiling may cause protein aggregation. Exposure time : 81 seconds.

All lanes:

Western blot - Anti-SCAP antibody [EPR26242-60] (<a href='/en-us/products/primary-antibodies/scap-antibody-epr26242-60-ab308060'>ab308060</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 whole cell lysate at 20 µg

Lane 2:

PC-12 whole cell lysate at 20 µg

Lane 3:

NIH/3T3 fresh whole cell lysate at 20 µg

Lane 4:

PC-12 fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa

false

Exposure time: 81s