Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free)

• IHC

Supplier Data

Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633)

This data was developed using ab300632, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular tissue labeling Scavenging Receptor SR-BI with ab300632 at 1/1000 (1.01 µg/mL) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human hepatocellular carcinoma. The section was incubated with ab300632 for 30mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633)

This data was developed using ab300632, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Scavenging Receptor SR-BI with ab300632 at 1/1000 (1.01 µg/mL) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on blood vessels of human cerebrum. The section was incubated with ab300632 for 30mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633)

This data was developed using ab300632, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Scavenging Receptor SR-BI with ab300632 at 1/1000 (1.01 µg/mL) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human liver. The section was incubated with ab300632 for 30mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633)

False colour image of Western blot : Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab300632 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70 and 75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line ab282646. To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution. This data was developed using ab300632, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (<a href='/en-us/products/primary-antibodies/scavenging-receptor-sr-bi-antibody-25-cla-1-ab300632'>ab300632</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human SCARB1 knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-scarb1-knockout-hek-293t-cell-lysate-ab283046'>ab283046</a>)

Lane 2:

Western blot - Human SCARB1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-scarb1-knockout-hek-293t-cell-line-ab282646'>ab282646</a>)

Lane 2:

SCARB1 knockout HEK-293T cell lysate at 20 µg

Lane 3:

Human Liver cell lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/20000 dilution

Lanes 1 - 3:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa,75 kDa

false

• WB

Supplier Data

Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633)

All lanes:

Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (<a href='/en-us/products/primary-antibodies/scavenging-receptor-sr-bi-antibody-25-cla-1-ab300632'>ab300632</a>) at 1/1000 dilution

Lane 1:

LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

Human liver tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

• WB

Supplier Data

Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633)

All lanes:

Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (<a href='/en-us/products/primary-antibodies/scavenging-receptor-sr-bi-antibody-25-cla-1-ab300632'>ab300632</a>) at 1/1000 dilution

Lane 1:

Wild type HAP1, whole cell lysate at 20 µg

Lane 2:

SCARB1 knockout HAP1, whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/10000 dilution

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

false