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Mouse Recombinant Monoclonal Scavenging Receptor SR-BI antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633), expandable thumbnail
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633), expandable thumbnail
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633), expandable thumbnail
  • Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633), expandable thumbnail
  • Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (AB300633), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PWB
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells (PubMed:12016218, PubMed:12519372, PubMed:21226579). Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux (PubMed:26965621). Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (PubMed:12016218). (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry (PubMed:12356718, PubMed:12913001, PubMed:18000990). Binding between SCARB1 and the hepatitis C virus glycoprotein E2 is independent of the genotype of the viral isolate (PubMed:12356718). (Microbial infection) Mediates uptake of M.fortuitum, E.coli and S.aureus. (Microbial infection) Facilitates the entry of human coronavirus SARS-CoV-2 by acting as an entry cofactor through HDL binding.

Alternative names

CD36, CD36L1, CLA1, SCARB1, Scavenger receptor class B member 1, SRB1, CD36 and LIMPII analogous 1, CD36 antigen-like 1, SR-BI, CLA-1

Recommended products

Mouse Recombinant Monoclonal Scavenging Receptor SR-BI antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
25/CLA-1 H1L1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Scavenging Receptor Class B Type 1 (SR-BI) also known as CLA-1 in humans is a membrane protein involved in the selective uptake of lipids. This protein has a molecular mass of approximately 82 kDa and is primarily expressed in the liver and non-placental tissues such as adrenal glands and ovaries. SR-BI mediates the transfer of cholesterol esters from high-density lipoprotein (HDL) to cells while playing a role in reverse cholesterol transport. Its function is essential for maintaining cholesterol homeostasis.

Biological function summary

SR-BI contributes to lipid metabolism by influencing the dynamics of cholesterol and lipid circulation within the body. It forms part of the larger HDL receptor complex which facilitates the selective uptake of lipids without internalizing the entire lipoprotein. Through this mechanism SR-BI assists cells in acquiring necessary components for membrane synthesis and hormone production.

Pathways

SR-BI integrates into the cholesterol biosynthesis pathway and the reverse cholesterol transport pathway. In these pathways SR-BI works closely with proteins like ATP-binding cassette transporter A1 (ABCA1) and lecithin-cholesterol acyltransferase (LCAT). These interactions promote the efflux and transport of cholesterol therefore playing a vital role in lipid regulation and metabolic processes.

Associated diseases and disorders

SR-BI's malfunction can lead to cardiovascular diseases due to its role in cholesterol metabolism. Elevated levels of HDL cholesterol without functional SR-BI impair reverse cholesterol transport potentially leading to atherosclerosis. Moreover SR-BI interacts with apolipoprotein A-I (apoA-I) a major protein component of HDL impacting its efficacy and connection to coronary artery disease. Understanding SR-BI pathways and their interactions can provide insights into therapeutic strategies for treating cholesterol-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633)

    False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70 and 75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line Human SCARB1 knockout HEK-293T cell line ab282646. To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.

    This data was developed using Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell lysate (Human SCARB1 knockout HEK-293T cell lysate ab283046)

    Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell line (Human SCARB1 knockout HEK-293T cell line ab282646)

    Lane 2: SCARB1 knockout HEK-293T cell lysate at 20 µg

    Lane 3: Human Liver cell lysate at 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution

    Lanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution

    Predicted band size: 60 kDa

    Observed band size: 70 kDa, 75 kDa

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633)

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632) at 1/1000 dilution

    Lane 1: Wild type HAP1, whole cell lysate at 20 µg

    Lane 2: SCARB1 knockout HAP1, whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 at 20 µg

    Lane 4: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/10000 dilution

    Lanes 1 - 4: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633)

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632) at 1/1000 dilution

    Lane 1: LNCaP (human prostate carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 3: Human liver tissue lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

  • Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633), expandable thumbnail

    Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633)

    This data was developed using Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular tissue labeling Scavenging Receptor SR-BI with Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632 at 1/1000 (1.01 µg/mL) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human hepatocellular carcinoma. The section was incubated with abxxxxxx for 30mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633), expandable thumbnail

    Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633)

    This data was developed using Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Scavenging Receptor SR-BI with Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632 at 1/1000 (1.01 µg/mL) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on blood vessels of human cerebrum. The section was incubated with abxxxxxx for 30mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633), expandable thumbnail

    Immunohistochemistry - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (BSA and Azide free) (ab300633)

    This data was developed using Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Scavenging Receptor SR-BI with Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632 at 1/1000 (1.01 µg/mL) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human liver. The section was incubated with abxxxxxx for 30mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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