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Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) is a rabbit monoclonal antibody detecting Scavenging Receptor SR-BI in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 50 publications

- Trusted since 2007


Images

Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB52629), expandable thumbnail
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB52629), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB52629), expandable thumbnail
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB52629), expandable thumbnail
  • Functional Studies - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (AB52629), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Tested
Expected
Rat
Not recommended
Not recommended
Tested
Expected
Sheep
Not recommended
Not recommended
Predicted
Predicted

Not recommended
Not recommended

Species
Mouse, Rat, Human, Sheep
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Sheep
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/2000
Notes

-

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Predicted
Predicted

Species
Sheep
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

1 product for Alternative Version

Target data

Function

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells (PubMed:12016218, PubMed:12519372, PubMed:21226579). Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux (PubMed:26965621). Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (PubMed:12016218). (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry (PubMed:12356718, PubMed:12913001, PubMed:18000990). Binding between SCARB1 and the hepatitis C virus glycoprotein E2 is independent of the genotype of the viral isolate (PubMed:12356718). (Microbial infection) Mediates uptake of M.fortuitum, E.coli and S.aureus. (Microbial infection) Facilitates the entry of human coronavirus SARS-CoV-2 by acting as an entry cofactor through HDL binding.

Alternative names

Recommended products

Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) is a rabbit monoclonal antibody detecting Scavenging Receptor SR-BI in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 50 publications

- Trusted since 2007

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1556Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

Trusted by the scientific community


Anti-Scavenging Receptor SR-BI [EP1556Y] (ab52629) was first used in a scientific publication in 2007 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) has been confirmed by Western blot testing in SCARB1 Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EP1556Y] also available for your convenience:
ab52629, Carrier free - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free ab176238, HRP - HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab206233, Carrier free - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - BSA and Azide free ab271844, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab311053, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab311179, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab313117, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab313310, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab321657



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Scavenging Receptor Class B Type 1 (SR-BI) also known as CLA-1 in humans is a membrane protein involved in the selective uptake of lipids. This protein has a molecular mass of approximately 82 kDa and is primarily expressed in the liver and non-placental tissues such as adrenal glands and ovaries. SR-BI mediates the transfer of cholesterol esters from high-density lipoprotein (HDL) to cells while playing a role in reverse cholesterol transport. Its function is essential for maintaining cholesterol homeostasis.

Biological function summary

SR-BI contributes to lipid metabolism by influencing the dynamics of cholesterol and lipid circulation within the body. It forms part of the larger HDL receptor complex which facilitates the selective uptake of lipids without internalizing the entire lipoprotein. Through this mechanism SR-BI assists cells in acquiring necessary components for membrane synthesis and hormone production.

Pathways

SR-BI integrates into the cholesterol biosynthesis pathway and the reverse cholesterol transport pathway. In these pathways SR-BI works closely with proteins like ATP-binding cassette transporter A1 (ABCA1) and lecithin-cholesterol acyltransferase (LCAT). These interactions promote the efflux and transport of cholesterol therefore playing a vital role in lipid regulation and metabolic processes.

Associated diseases and disorders

SR-BI's malfunction can lead to cardiovascular diseases due to its role in cholesterol metabolism. Elevated levels of HDL cholesterol without functional SR-BI impair reverse cholesterol transport potentially leading to atherosclerosis. Moreover SR-BI interacts with apolipoprotein A-I (apoA-I) a major protein component of HDL impacting its efficacy and connection to coronary artery disease. Understanding SR-BI pathways and their interactions can provide insights into therapeutic strategies for treating cholesterol-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HepG2 whole cell lysate (20 μg)
    Lane 4: Human liver whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. ab52629 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    Predicted band size: 60 kDa

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution

    Lane 1: Mouse liver lysate at 20 µg

    Lane 2: Rat liver lysate at 20 µg

    Secondary

    All lanes: Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 60 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 20 µg

    Lane 2: HepG2 lysate at 20 µg

    Lane 3: PC-3 cell lysate at 20 µg

    Secondary

    All lanes: Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 60 kDa

    Observed band size: 80 kDa

  • Functional Studies - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), expandable thumbnail

    Functional Studies - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    THP1 cells were incubated at 37°C for 40h with vehicle control (0 μM) and different concentrations of sodium salicylate (Sodium salicylate, anti-inflammatory agent ab120746). Decreased expression of scavenging receptor SR-BI in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab52629 at 1/2000 dilution and Anti-beta Actin antibody - Loading Control ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/2000 dilution

    All lanes: Mouse liver lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa

    Observed band size: 80 kDa

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), expandable thumbnail

    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EP1556Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52629 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line Human SCARB1 knockout HEK-293T cell line ab282646 (knockout cell lysate Human SCARB1 knockout HEK-293T cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: SCARB1 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell line (Human SCARB1 knockout HEK-293T cell line ab282646)

    Lane 3: Human Liver cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Observed band size: 70 kDa, 75 kDa

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Product protocols

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