Rabbit Recombinant Monoclonal Scavenging Receptor SR-BI antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (AB176238), expandable thumbnail](https://content.abcam.com/products/images/scavenging-receptor-sr-bi-antibody-ep1556y-low-endotoxin-azide-free-ab176238--western-blot-img137069.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (AB176238)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (AB176238), expandable thumbnail](https://content.abcam.com/products/images/scavenging-receptor-sr-bi-antibody-ep1556y-low-endotoxin-azide-free-ab176238--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111701.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (AB176238)")
![Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (AB176238), expandable thumbnail](https://content.abcam.com/products/images/scavenging-receptor-sr-bi-antibody-ep1556y-low-endotoxin-azide-free-ab176238--western-blot-img164538.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (AB176238)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Tested | Expected |
| Rat | Not recommended | Not recommended | Tested | Expected |
| Sheep | Not recommended | Not recommended | Predicted | Predicted |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Sheep | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Sheep | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
4 products for Alternative Version
Target data
Function
Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells (PubMed:12016218, PubMed:12519372, PubMed:21226579). Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux (PubMed:26965621). Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (PubMed:12016218). (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry (PubMed:12356718, PubMed:12913001, PubMed:18000990). Binding between SCARB1 and the hepatitis C virus glycoprotein E2 is independent of the genotype of the viral isolate (PubMed:12356718). (Microbial infection) Mediates uptake of M.fortuitum, E.coli and S.aureus. (Microbial infection) Facilitates the entry of human coronavirus SARS-CoV-2 by acting as an entry cofactor through HDL binding.
Alternative names
CD36, CD36L1, CLA1, SCARB1, Scavenger receptor class B member 1, SRB1, CD36 and LIMPII analogous 1, CD36 antigen-like 1, SR-BI, CLA-1
Recommended products
Rabbit Recombinant Monoclonal Scavenging Receptor SR-BI antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1556Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab176238 is the carrier-free version of Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Scavenging Receptor Class B Type 1 (SR-BI) also known as CLA-1 in humans is a membrane protein involved in the selective uptake of lipids. This protein has a molecular mass of approximately 82 kDa and is primarily expressed in the liver and non-placental tissues such as adrenal glands and ovaries. SR-BI mediates the transfer of cholesterol esters from high-density lipoprotein (HDL) to cells while playing a role in reverse cholesterol transport. Its function is essential for maintaining cholesterol homeostasis.
- Biological function summary
SR-BI contributes to lipid metabolism by influencing the dynamics of cholesterol and lipid circulation within the body. It forms part of the larger HDL receptor complex which facilitates the selective uptake of lipids without internalizing the entire lipoprotein. Through this mechanism SR-BI assists cells in acquiring necessary components for membrane synthesis and hormone production.
- Pathways
SR-BI integrates into the cholesterol biosynthesis pathway and the reverse cholesterol transport pathway. In these pathways SR-BI works closely with proteins like ATP-binding cassette transporter A1 (ABCA1) and lecithin-cholesterol acyltransferase (LCAT). These interactions promote the efflux and transport of cholesterol therefore playing a vital role in lipid regulation and metabolic processes.
- Associated diseases and disorders
SR-BI's malfunction can lead to cardiovascular diseases due to its role in cholesterol metabolism. Elevated levels of HDL cholesterol without functional SR-BI impair reverse cholesterol transport potentially leading to atherosclerosis. Moreover SR-BI interacts with apolipoprotein A-I (apoA-I) a major protein component of HDL impacting its efficacy and connection to coronary artery disease. Understanding SR-BI pathways and their interactions can provide insights into therapeutic strategies for treating cholesterol-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238), expandable thumbnail](https://content.abcam.com/products/images/scavenging-receptor-sr-bi-antibody-ep1556y-low-endotoxin-azide-free-ab176238--western-blot-img137069.jpg "Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)")
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 μg)
Lane 3: HepG2 whole cell lysate (20 μg)
Lane 4: Human liver whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176238).
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629)
Predicted band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238), expandable thumbnail](https://content.abcam.com/products/images/scavenging-receptor-sr-bi-antibody-ep1556y-low-endotoxin-azide-free-ab176238--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111701.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
Immunohistochemical staining of paraffin embedded human liver with purified Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629 at a working dilution of 1/500. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629).
![Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238), expandable thumbnail](https://content.abcam.com/products/images/scavenging-receptor-sr-bi-antibody-ep1556y-low-endotoxin-azide-free-ab176238--western-blot-img164538.jpg "Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)")
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EP1556Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line Human SCARB1 knockout HEK-293T cell line ab282646 (knockout cell lysate Human SCARB1 knockout HEK-293T cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SCARB1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell line (Human SCARB1 knockout HEK-293T cell line ab282646)
Lane 3: Human Liver cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 70 kDa, 75 kDa
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