Name: Anti-Scavenging Receptor SR-BI antibody [EPR20190]
SKU: ab217318
Rating: 5 (2 reviews)

Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) is a rabbit monoclonal antibody detecting Scavenging Receptor SR-BI in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 40 publications

Images

Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (AB217318), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Tested
Rat	Expected	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

See full target information SCARB1

Function

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells (PubMed:12016218, PubMed:12519372, PubMed:21226579). Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux (PubMed:26965621). Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (PubMed:12016218). (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry (PubMed:12356718, PubMed:12913001, PubMed:18000990). Binding between SCARB1 and the hepatitis C virus glycoprotein E2 is independent of the genotype of the viral isolate (PubMed:12356718). (Microbial infection) Mediates uptake of M.fortuitum, E.coli and S.aureus. (Microbial infection) Facilitates the entry of human coronavirus SARS-CoV-2 by acting as an entry cofactor through HDL binding.

Alternative names

CD36, CD36L1, CLA1, SCARB1, Scavenger receptor class B member 1, SRB1, CD36 and LIMPII analogous 1, CD36 antigen-like 1, SR-BI, CLA-1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20190
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Scavenging Receptor SR-BI?

Anti-Scavenging Receptor SR-BI [EPR20190] (ab217318) specifically detects a band for Scavenging Receptor SR-BI (UniProt: Q8WTV0) at a molecular weight of 60kDa.

Trusted by the scientific community

Anti-Scavenging Receptor SR-BI [EPR20190] (ab217318) was first used in a scientific publication in 2017 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) has been confirmed by Western blot testing in SCARB1 Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EPR20190] also available for your convenience:
ab217318, Carrier free - ab222931, Carrier free - ab272003

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Scavenging Receptor Class B Type 1 (SR-BI) also known as CLA-1 in humans is a membrane protein involved in the selective uptake of lipids. This protein has a molecular mass of approximately 82 kDa and is primarily expressed in the liver and non-placental tissues such as adrenal glands and ovaries. SR-BI mediates the transfer of cholesterol esters from high-density lipoprotein (HDL) to cells while playing a role in reverse cholesterol transport. Its function is essential for maintaining cholesterol homeostasis.

Biological function summary

SR-BI contributes to lipid metabolism by influencing the dynamics of cholesterol and lipid circulation within the body. It forms part of the larger HDL receptor complex which facilitates the selective uptake of lipids without internalizing the entire lipoprotein. Through this mechanism SR-BI assists cells in acquiring necessary components for membrane synthesis and hormone production.

Pathways

SR-BI integrates into the cholesterol biosynthesis pathway and the reverse cholesterol transport pathway. In these pathways SR-BI works closely with proteins like ATP-binding cassette transporter A1 (ABCA1) and lecithin-cholesterol acyltransferase (LCAT). These interactions promote the efflux and transport of cholesterol therefore playing a vital role in lipid regulation and metabolic processes.

Associated diseases and disorders

SR-BI's malfunction can lead to cardiovascular diseases due to its role in cholesterol metabolism. Elevated levels of HDL cholesterol without functional SR-BI impair reverse cholesterol transport potentially leading to atherosclerosis. Moreover SR-BI interacts with apolipoprotein A-I (apoA-I) a major protein component of HDL impacting its efficacy and connection to coronary artery disease. Understanding SR-BI pathways and their interactions can provide insights into therapeutic strategies for treating cholesterol-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 μg)
Lane 3: HepG2 whole cell lysate (20 μg)
Lane 4: Human liver whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab217318 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab217318 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. ab217318 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Predicted band size: 60 kDa
Immunoprecipitation - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Scavenging Receptor SR-BI was immunoprecipitated from 0.35 mg of Human fetal liver lysate with ab217318 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217318 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human fetal liver lysate, 10 μg (Input).
Lane 2: ab217318 IP in Human fetal liver lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab217318 in Human fetal liver lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Predicted band size: 60 kDa
Observed band size: 82 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Immunofluorescent analysis of 100% methanol fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Scavenging Receptor SR-BI with ab217318 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HepG2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EPR20190] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab217318 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line Human SCARB1 knockout HEK-293T cell line ab282646 (knockout cell lysate Human SCARB1 knockout HEK-293T cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SCARB1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell line (Human SCARB1 knockout HEK-293T cell line ab282646)
Lane 3: Human Liver cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 70 kDa, 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 4 seconds; Lane 4-6: 3 minutes.
Scavenging Receptor SR-BI undergoes N-glycosylation when it is expressed. The molecular weight observed is consistent with what has been described in the literature (PMID: 12016218; PMID: 10821819; PMID: 224442701).
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution
Lane 1: Human fetal liver lysate at 20 µg
Lane 2: Mouse liver lysate at 20 µg
Lane 3: Rat liver lysate at 20 µg
Lane 4: Mouse heart lysate at 10 µg
Lane 5: Mouse kidney lysate at 10 µg
Lane 6: Mouse spleen lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 82 kDa
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 30 seconds; Lane 2: 4 seconds; Lane 3: 2 seconds; Lane 4-5: 5 seconds.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution
Lane 1: U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg
Lane 2: LNCaP (Human prostate cancer cell line) whole cell lysate at 10 µg
Lane 3: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 4: THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg
Lane 5: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 82 kDa
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution
Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 2: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 82 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human diffuse large B cell lymphoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on rat liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on rat cerebral cortex blood vessel endothelium is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.