Rabbit Polyclonal SCC112 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDS5A aa 1250 to C-terminus.
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Publications
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- Molecular cell 73:212-223.e72018Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCornelia Meisenberg,Sarah I Pinder,Suzanna R Hopkins,Sarah K Wooller,Graeme Benstead-Hume,Frances M G Pearl,Penny A Jeggo,Jessica A DownsPubMed 30554942
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PDS5A aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q29RF7
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Alternative names
KIAA0648, PDS5, PIG54, PDS5A, Sister chromatid cohesion protein PDS5 homolog A, Cell proliferation-inducing gene 54 protein, Sister chromatid cohesion protein 112, SCC-112
Recommended products
Rabbit Polyclonal SCC112 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDS5A aa 1250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PDS5A aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q29RF7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCC112 also known as LAP1B is a protein involved in cellular structure and function processes. It has a molecular weight of approximately 75 kDa. SCC112 is expressed in various tissues including liver kidney and muscle. The protein is localized mainly to the inner nuclear membrane where it plays roles in maintaining nuclear structure and function. This localization position allows SCC112 to interact with a range of other nuclear proteins showing its involvement in broader cellular processes.
- Biological function summary
The role of SCC112 is linked to its ability to regulate nuclear architecture and chromatin organization. It is part of the nuclear lamina-associated polypeptide family and is involved in complex formations with proteins responsible for maintaining nuclear stability. SCC112 influences the dynamics of chromatin interactions which impacts gene expression. Through these complex formations SCC112 helps in organizing the nuclear architecture which has downstream effects on various cellular activities.
- Pathways
SCC112 acts within the nuclear envelope dynamics and chromatin organization pathways. It closely interacts with lamin proteins such as LMNA and LMNB1 which are essential for nuclear stability. These interactions facilitate the cohesion of nuclear structures and are necessary for appropriate chromatin organization. The involvement of SCC112 in these pathways suggests a broad influence on cellular replication and gene regulation. The precise regulation of chromatin architecture by SCC112 plays a significant part in cellular division processes and the maintenance of cellular integrity.
- Associated diseases and disorders
SCC112 has been associated with muscular dystrophy and certain types of cancer. Alterations in SCC112 expression levels can impact the balance of nuclear stability contributing to diseases such as Emery-Dreifuss muscular dystrophy where LMNA mutations are prevalent. Also abnormalities in SCC112 have been noted in certain cancer types suggesting a possible role in tumor progression. The connection with these diseases highlights the significant influence of SCC112 on nuclear integrity and cellular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SCC112 antibody (ab122352)
Immunocytochemistry analysis of U-251 MG (formally U-373 MG) (human brain glioma cell line) cells labelling SCC112 with ab122352 at 2 μg/mL.
Western blot - Anti-SCC112 antibody (ab122352)
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)All lanes: Western blot - Anti-SCC112 antibody (ab122352)
Predicted band size: 151 kDa
Western blot - Anti-SCC112 antibody (ab122352)
All lanes: Western blot - Anti-SCC112 antibody (ab122352) at 0.4 µg/mL
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (formally U-373 MG) (human brain glioma cell line) cell lysate
Predicted band size: 151 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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